{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T10:25:53Z","timestamp":1770719153629,"version":"3.49.0"},"reference-count":21,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2005,11,22]],"date-time":"2005-11-22T00:00:00Z","timestamp":1132617600000},"content-version":"vor","delay-in-days":11832,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1973,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A dilution scheme for bone marrow\u2010derived (B) cells has been applied to estimate the frequency of precursor cells specific to sheep red cells (SRC) present in the spleens of nude mice.<\/jats:p><jats:p>B cells were titrated over a range of 0.2 x 10<jats:sup>5<\/jats:sup> \u2013 1.8 x 10<jats:sup>5<\/jats:sup> spleen cells of nude mice per microculture in the presence of a constant number of allogeneic spleen cells. Data from 6 limiting dilution experiments are presented. It is argued that only the B cells are being diluted out. The frequency of precursor cells specific for SRC was calculated by means of Poisson statistics. The estimates of these frequencies are in the range 0.9 x 10<jats:sup>\u20105<\/jats:sup> \u2013 2 x 10<jats:sup>\u20105<\/jats:sup>.<\/jats:p>","DOI":"10.1002\/eji.1830030704","type":"journal-article","created":{"date-parts":[[2007,2,27]],"date-time":"2007-02-27T23:11:33Z","timestamp":1172617893000},"page":"392-397","source":"Crossref","is-referenced-by-count":81,"title":["Precursor cells specific to sheep red cells in nude mice. estimation of frequency in the microculture system"],"prefix":"10.1002","volume":"3","author":[{"given":"J.","family":"Quint\u00e1ns","sequence":"first","affiliation":[]},{"given":"I.","family":"Lefkovits","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,11,22]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830020412"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1159\/000313169"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-140-36500"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830030705"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/icb.1972.57"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.61.2.542"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(71)90002-5"},{"key":"e_1_2_1_9_2","first-page":"96","volume-title":"Statistical Methods for Research Workers","author":"Fisher R. 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