{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,10]],"date-time":"2023-11-10T23:57:38Z","timestamp":1699660658828},"reference-count":23,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,11,22]],"date-time":"2005-11-22T00:00:00Z","timestamp":1132617600000},"content-version":"vor","delay-in-days":10521,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1977,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Populations of lymphocytes primed to alloantigens <jats:italic>in vitro<\/jats:italic> are stimulated by allogeneic cells which are unrelated to the specific stimulator cells used for priming. The number of lymphocytes in the primed population which responded to unrelated stimulators could be estimated in relation to the number of primed lymphocytes responding to the specific stimulator. This was done by measuring the responses of decreasing numbers of primed lymphocytes to various stimulator cell populations. Similar estimates were obtained on several occasions using the same combination of lymphocytes which were primed and retested at different times.<\/jats:p><jats:p>There was a close correlation between the estimated frequency of lymphocytes in a primed population which responded to unrelated stimulators and the number of responder lymphocytes required to obtain a measurable response. Stimulators which activated a high frequency of primed cells required fewer responder lymphocytes than those which activated lower frequencies of primed cells. The specific stimulator was capable of initiating a response using fewer responder lymphocytes than were most unrelated stimulator cells. This confirms that primed populations are predominantly enriched for lymphocytes responding to the specific stimulator.<\/jats:p><jats:p>At least some lymphocytes in a primed population responded to both the specific stimulator and unrelated stimulator cells. This was demonstrated in two types of experiments. In the first, it was possible to enrich lymphocytes in a primed population which responded to unrelated stimulator cells without depleting lymphocytes which also responded to the specific stimulator. In the second, primed lymphocytes from donor A, which showed strong reactivity to stimulators from donor D after priming with B stimulator cells, also showed significant reactivity to B stimulators after priming with D cells. These results strongly suggest that primed populations contain several subpopulations (clones) of lymphocytes whose specificities differ as exhibited by their responsiveness to unrelated stimulator cells.<\/jats:p>","DOI":"10.1002\/eji.1830070208","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T05:01:25Z","timestamp":1172638885000},"page":"93-99","source":"Crossref","is-referenced-by-count":19,"title":["Responses of alloantigen\u2010primed lymphocytes in vitro Quantitative analysis of the relative frequency of reactive lymphocytes in primed populations which respond to allogeneic stimulating cells"],"prefix":"10.1002","volume":"7","author":[{"given":"R. B.","family":"Corley","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,22]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1974.tb01318.x"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.140.3.718"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.140.3.660"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.124948"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.1976.tb01057.x"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01576936"},{"key":"e_1_2_1_8_2","first-page":"1005","volume":"1976","author":"Alter B.J.","journal-title":"J. Exp. Med."},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830050502"},{"key":"e_1_2_1_10_2","unstructured":"Corley R.B.andZimmermann R. 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