{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T07:05:52Z","timestamp":1762067152194},"reference-count":13,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,12,6]],"date-time":"2005-12-06T00:00:00Z","timestamp":1133827200000},"content-version":"vor","delay-in-days":10170,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1978,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The MLC\u2010CML reaction depends on cooperation between different subsets of T lymphocytes in which soluble mediators may play a critical role. Evidence is presented that cell\u2010free supernatants from mixed cultures, containing blastogenic factor (BF) activity can, together with solubilized membrane preparations, induce a primary cell\u2010mediated lympholysis (CML) reaction of the same order of magnitude as intact allogeneic stimulating cells. Furthermore, BF alone can induce secondary mixed leukocyte culture (MLC) and CML reactions very efficiently. The results described suggest that BF is produced in response to activation by LD determinants, probably by helper T cells, and that the target of BF is the cytotoxic lymphocyte. It would appear that regulation of CML activity may be mediated by BF, and exhaustion of the mediator may be responsible for the decline of cell proliferation and effector function in the MLC reaction.<\/jats:p>","DOI":"10.1002\/eji.1830080212","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T09:36:45Z","timestamp":1172655405000},"page":"133-138","source":"Crossref","is-referenced-by-count":22,"title":["Blastogenic factor: its role in the mixed leukocyte culture reaction"],"prefix":"10.1002","volume":"8","author":[{"given":"Marjatta","family":"Uotila","sequence":"first","affiliation":[]},{"given":"H. N.","family":"Rode","sequence":"additional","affiliation":[]},{"given":"J.","family":"Gordon","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,12,6]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/208795a0"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/208794a0"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(71)90003-7"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-597850-7.50019-5"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/260329a0"},{"issue":"21","key":"e_1_2_1_7_2","first-page":"77","volume":"97","author":"B\u00f8yum A.","year":"1968","journal-title":"Scand. J. Immunol."},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.153.3735.545"},{"key":"e_1_2_1_9_2","unstructured":"Chan E. S. Studies on a \u2018Blastogenic Factor\u2019 Produced by Leukocytes in Mixed Culture M.Sc. Thesis McGill University Montreal1970 p.74."},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-196709000-00010"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.172.3988.1134"},{"key":"e_1_2_1_12_2","first-page":"335","volume-title":"Proceedings of the Fourth Annual Leukocyte Culture Conference","author":"Janis M.","year":"1971"},{"key":"e_1_2_1_13_2","first-page":"125","volume":"29","author":"Eijsvoogel V. P.","year":"1976","journal-title":"Transplant. Rev."},{"key":"e_1_2_1_14_2","doi-asserted-by":"crossref","first-page":"1824","DOI":"10.4049\/jimmunol.117.5_Part_2.1824","volume":"117","author":"Geczy C. L.","year":"1976","journal-title":"J. Immunol."}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Feji.1830080212","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.1830080212","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,2]],"date-time":"2023-11-02T12:14:57Z","timestamp":1698927297000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.1830080212"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1978,2]]},"references-count":13,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1978,2]]}},"alternative-id":["10.1002\/eji.1830080212"],"URL":"https:\/\/doi.org\/10.1002\/eji.1830080212","archive":["Portico"],"relation":{},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[1978,2]]}}}