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Binding of antibody to primary cells and cell lines was detected by radioimmune indirect binding assay, autoradiography or fluorescence\u2010activated cell sorter analysis. F4\/80 binds to mouse M\u03a6 from the peritoneal cavity or other sources, blood monocytes, M\u03a6 derived from bone marrow precursors in culture and M\u03a6\u2010like cell lines, but not to other cells, including polymorphonuclear leukocytes, lymphocytes or fibroblasts. F4\/80 does not bind to M\u03a6 via Fc receptors, is not cytotoxic and is of the rat IgG<jats:sub>2b<\/jats:sub> subclass. Since F4\/80 binds to all M\u03a6 defined by adherence, morphology and immune phagocytosis, it provides a new marker to define the M\u03a6 in the mouse. Large differences in expression of antigen F4\/80 were found, depending on intraperitoneal stimulation, time in culture and stage of maturation. Immunoprecipitation experiments demonstrated that the antigen F4\/80 is part of a component of Mr 160000 which is synthesized by the M\u03a6 and, at least in part, exposed on the cell surface.<\/jats:p>","DOI":"10.1002\/eji.1830111013","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T13:25:35Z","timestamp":1172669135000},"page":"805-815","source":"Crossref","is-referenced-by-count":1191,"title":["F4\/80, a monoclonal antibody directed specifically against the mouse macrophage"],"prefix":"10.1002","volume":"11","author":[{"given":"Jonathan M.","family":"Austyn","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siamon","family":"Gordon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,11,16]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.46.070177.003321"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0968-0004(80)90238-8"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-009-8793-7_26"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.152.5.1248"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.150.3.580"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830040207"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.147.4.1175"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.150.2.231"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"898","DOI":"10.4049\/jimmunol.114.2_Part_2.898","volume":"114","author":"Ralph P.","year":"1975","journal-title":"J. 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