{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T22:30:57Z","timestamp":1766788257757,"version":"build-2065373602"},"reference-count":23,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,4,13]],"date-time":"2005-04-13T00:00:00Z","timestamp":1113350400000},"content-version":"vor","delay-in-days":5216,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[1991,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A highly sensitive method for protein visualization following electrophoresis and protein blotting was developed. The method is based on the light\u2010emitting reaction of luminol and hydrogen peroxide catalyzed by horseradish peroxidase. The luminescent assay can be applied either to the native gel or after protein blotting, and it has a sensitivity two orders of magnitude higher than that achieved with chromogenic detection systems. Analogous to autoradiography the luminescent signal is recorded on an X\u2010ray film with similar sensitivity. We present several examples of application emphasizing the general versatility of this innovative method.<\/jats:p>","DOI":"10.1002\/elps.1150120508","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T09:23:05Z","timestamp":1117272185000},"page":"367-372","source":"Crossref","is-referenced-by-count":62,"title":["Luminography \u2013 a new, highly sensitive visualization method for electrophoresis"],"prefix":"10.1002","volume":"12","author":[{"given":"Reinhard","family":"Schneppenheim","sequence":"first","affiliation":[]},{"given":"Ulrich","family":"Budde","sequence":"additional","affiliation":[]},{"given":"Nicolaus","family":"Dahlmann","sequence":"additional","affiliation":[]},{"given":"Peter","family":"Rautenberg","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,4,13]]},"reference":[{"volume-title":"Analytical Applications of Bioluminescence and Chemiluminescence","year":"1984","author":"Kricka L. 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