{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T17:28:20Z","timestamp":1772818100942,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,14]],"date-time":"2006-07-14T00:00:00Z","timestamp":1152835200000},"content-version":"vor","delay-in-days":5673,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Environ and Mol Mutagen"],"published-print":{"date-parts":[[1991,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A rapid method for determining the DNA sequences of <jats:italic>Salmonella typhimurium hisD3052<\/jats:italic> revertants is presented. DNA colony hybridization was used to analyze revertants previously studied by Isono and Yourno [Proc Natl Acad Sci USA 71:1612\u20131617, 1974]. Synthetic oligodeoxy\u2010ribonucleotide probes (18\u2010mers) were able to distinguish sequences that differed by a single base pair. Mutant <jats:italic>his<\/jats:italic> sequences not identified by probing analysis were amplified using polymerase chain reaction (PCR) and directly sequenced. The combined use of DNA\u2010colony hybridization and direct sequencing offers a precise and rapid means for the molecular characterization of <jats:italic>hisD3052<\/jats:italic> revertants.<\/jats:p>","DOI":"10.1002\/em.2850180404","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T18:36:34Z","timestamp":1172082994000},"page":"224-230","source":"Crossref","is-referenced-by-count":35,"title":["Analysis of <i>Salmonella typhimurium hisD3052<\/i> revertants: The use of oligodeoxyribonucleotide colony hybridization, PCR, and direct sequencing in mutational analysis"],"prefix":"10.1002","volume":"18","author":[{"given":"Eugene","family":"Kupchella","sequence":"first","affiliation":[]},{"given":"Thomas A.","family":"Cebula","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2006,7,14]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.69.11.3128"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.176.4030.47"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.70.3.782"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1161(91)90249-8"},{"key":"e_1_2_1_6_1","first-page":"349","volume-title":"Metabolic Pathways","author":"Brenner M","year":"1971"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(88)90194-5"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/18.13.4028"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/em.2860080504"},{"key":"e_1_2_1_10_1","unstructured":"CebulaTA PayneWL TrucksessMW HillWE(1987): Colony probing ofSalmonella typhimurium hisD3052 revertants. 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