{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T10:19:07Z","timestamp":1758709147831},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,7,18]],"date-time":"2006-07-18T00:00:00Z","timestamp":1153180800000},"content-version":"vor","delay-in-days":14370,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1967,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>From a single primary culture of pooled hamster embryos, two morphologically distinct, malignant cell lines have evolved in the absence of experimentally applied selective factors, chemical, physical or viral. The cells of both sublines are male. One subline, designated Nil\u20101, is extremely pleomorphic with lobulated nuclei and micronuclei. It was tumorigenic at the 35th passage (18 weeks) after initiation of the primary culture. The second subline, designated Nil\u20102, is fibroblastic and required 30\u201345 more subcultures than Nil\u20101 to become tumorigenic. The malignant properties of Nil\u20101 and Nil\u20102 were not correlated with any specific chromosome changes. At a time when Nil\u20102 cells were still sensitive to contact inhibition and were not tumorigenic, they could be transformed by polyoma virus but not by SV40. The parent primary culture, however, was susceptible to transformation by SV40.<\/jats:p><jats:p>Neither subline contained SV5 or lymphocytic choriomeningitis viral antigen or specific complement fixing antigen induced by polyoma virus, SV40, Rous sarcoma virus or adenovirus types 7, 12, 18, 21, or 31. No cytopathogenic agent and no mycoplasma could be isolated from the parent culture or sublires; no new transplantation antigen could be demonstrated. The possibilities in the etiology of the transformation are discussed.<\/jats:p>","DOI":"10.1002\/ijc.2910020209","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T12:38:55Z","timestamp":1171888735000},"page":"143-152","source":"Crossref","is-referenced-by-count":99,"title":["Two spontaneously transformed cell lines derived from the same hamster embryo culture"],"prefix":"10.1002","volume":"2","author":[{"given":"Leila","family":"Diamond","sequence":"first","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2006,7,18]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1964.18"},{"key":"e_1_2_1_3_1","first-page":"641","article-title":"In vitro transformation of normal cells to tumor cells by carcinogenic hydrocarbons","volume":"35","author":"Berwald Y.","year":"1965","journal-title":"J. nat. Cancer Inst."},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.48.1.41"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1159\/000386007"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1030660313"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(63)90058-8"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1030660206"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.99.2.167"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(64)90022-4"},{"key":"e_1_2_1_11_1","first-page":"477","article-title":"Spontaneous, malignant transformation of hamster embryo cells in vitro","volume":"36","author":"Gotlieb\u2010Stematsky T.","year":"1966","journal-title":"J. nat. Cancer Inst."},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1093\/oxfordjournals.bmb.a070381","article-title":"Immunology of the cancer cell: tumour\u2010specific antigens","volume":"21","author":"Haddow A.","year":"1965","journal-title":"Brit. med. Bull."},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(61)90192-6"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.50.2.379"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.52.1.53"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1101\/SQB.1962.027.001.044"},{"key":"e_1_2_1_17_1","first-page":"639","article-title":"Karyotype of the Syrian hamster","volume":"31","author":"Lehman J. M.","year":"1963","journal-title":"J. nat Cancer Inst."},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/2101343a0"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(62)90290-8"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.120.2.121"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/2011349a0"},{"key":"e_1_2_1_22_1","first-page":"191","article-title":"Effects of X\u2010irradiated feeder layers on mitotic activity and development of aneuploidy in mouse\u2010embryo cells in vitro","volume":"5","author":"Rothfels K. H.","year":"1963","journal-title":"Canad. Cancer Conf."},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(08)60556-2"},{"key":"e_1_2_1_24_1","doi-asserted-by":"crossref","first-page":"320","DOI":"10.4049\/jimmunol.88.3.320","article-title":"Application of a microtechnique to viral serological investigations","volume":"88","author":"Sever J. L.","year":"1962","journal-title":"J. Immunol."},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/2031355a0"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.17.2.299"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-116-29346"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1620\/tjem.86.380"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1620\/tjem.88.171"}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fijc.2910020209","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.2910020209","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,12]],"date-time":"2023-11-12T04:24:12Z","timestamp":1699763052000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.2910020209"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1967,3,15]]},"references-count":28,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1967,3,15]]}},"alternative-id":["10.1002\/ijc.2910020209"],"URL":"https:\/\/doi.org\/10.1002\/ijc.2910020209","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[1967,3,15]]}}}