{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T06:00:55Z","timestamp":1786168855562,"version":"3.56.0"},"reference-count":21,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,7,18]],"date-time":"2006-07-18T00:00:00Z","timestamp":1153180800000},"content-version":"vor","delay-in-days":14186,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1967,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The development and cultivation of two cell lines from human mesenchymal tumours is described. Lines 2T and 4T differed from normal human fibroblastic cell strains by showing cytological atypia (high nucleocytoplasmic ratio, hyperchromasia and pleo\u2010morphism), an altered \u201cepithelial\u2010like\u201d pavement growth pattern, a deficient inhibition of cell division in crowded cultures and a potential for \u201cindefinite\u201d multiplication in vitro. Both lines were chromosomally abnormal in all samples and differed karyo\u2010typically from any known continuous cell line as well as from each other. Line 2T has retained, at least for 3 months (passage 111\u2013126), a fairly narrow chromosomal mode of 34\u201338 with two specific marker chromosomes. A high frequency of chromosome breakage was observed in line 2T and extensive chromosome fragmentation, \u201cchromosome pulverization\u201d, occurred. No viruses could, however, be detected in the cultures despite numerous attempts. A control cell strain (2S) was developed from uninvolved skin of one of the tumour patients. Its behaviour followed the general pattern of human diploid cell strains in culture. To our knowledge, the described cell lines are the first derived from human mesenchymal tumours where the availability of a control fibroblast strain will provide us with opportunities to compare autologous normal and neoplastic human mesenchymal cells under in vitro conditions.<\/jats:p>","DOI":"10.1002\/ijc.2910020505","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T13:06:43Z","timestamp":1171890403000},"page":"434-447","source":"Crossref","is-referenced-by-count":277,"title":["Two established in vitro cell lines from human mesenchymal tumours"],"prefix":"10.1002","volume":"2","author":[{"given":"Jan","family":"Ponten","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eero","family":"Saksela","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,18]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"556","article-title":"Isolation and long\u2010term culture of diploid mammalian cell lines","volume":"22","author":"Ferguson J.","year":"1962","journal-title":"Cancer Res."},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-117-29731"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(62)90061-2"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(65)90211-9"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(61)90192-6"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.50.2.379"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(64)90231-9"},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","unstructured":"Macieira\u2010Coelho A. Dissociation between inhibition of movement and inhibition of division in RSV transformed fibroblasts.Exp. Cell Res. in press 1967.","DOI":"10.1016\/0014-4827(67)90222-4"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(66)90373-9"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(196401)17:1<11::AID-CNCR2820170103>3.0.CO;2-E"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1030620109"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-5223.1965.tb02008.x"},{"key":"e_1_2_1_14_1","unstructured":"Opatija Conference for the characterization and uses of human diploid cell strains. Sept. 1963. Minimum requirements for the selection and use of human diploid cell strains in the production of virus vaccines in:Proceedings of the Conference Publ. Inst. Immunol. Zagreb 709\u2013733(1964)."},{"key":"e_1_2_1_15_1","unstructured":"Pont\u00e9n J. andMacieira\u2010Coelho A. Contact inhibition and cell cycle inhibition in cell cultures derived from normal and neoplastic mesenchyme in preparation (1967)."},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1159\/000129733"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.50.2.390"},{"key":"e_1_2_1_18_1","first-page":"132","article-title":"Chromosomal damage of human cells induced by the Sendai virus","volume":"43","author":"Saksela E.","year":"1965","journal-title":"Ann. Med. exp. Fenn."},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(08)60556-2"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(64)90253-3"},{"key":"e_1_2_1_21_1","doi-asserted-by":"crossref","first-page":"320","DOI":"10.4049\/jimmunol.88.3.320","article-title":"Application of a microtechnique to viral serological investigations","volume":"88","author":"Sever J. L.","year":"1962","journal-title":"J. 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