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Elevated levels of specific transferrin binding were found in the tumors with a range of 11\u201335% of bound transferrin, whereas microsomes prepared from non\u2010neoplastic breast tissue samples bound only 2.3% and 2.4% of the transferrin. Scatchard analysis of binding studies conducted with tissues from a breast cancer and from a breast sarcoma indicate that the receptor has a Ka = 9.0 \u00d7 10<jats:sup>8<\/jats:sup>M. The binding site is specific for transferrin, as studies show that non\u2010radioactive transferrin displaced labelled transferrin, while human IgG and human albumin did not. The receptor\u2010transferrin complex was precipitated from a detergent extract of the breast sarcoma with antiserum to human transferrin. Sodium dodecyl sulfate\/polyacrylamide gel electrophoresis of the immunoprecipitate gave a polypeptide of <jats:italic>M<\/jats:italic><jats:sub>r<\/jats:sub> 90,000 daltons, which is of similar molecular weight found for the putative transferrin receptor in all of a series of human cultured cell lines previously examined.<\/jats:p>","DOI":"10.1002\/ijc.2910270311","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T18:15:51Z","timestamp":1171908951000},"page":"329-334","source":"Crossref","is-referenced-by-count":155,"title":["Demonstration of the transferrin receptor in human breast cancer tissue. Potential marker for identifying dividing cells"],"prefix":"10.1002","volume":"27","author":[{"given":"Jeffrey E.","family":"Shindelman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann E.","family":"Ortmeyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Howard H.","family":"Sussman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,18]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"291","volume-title":"Proteins of iron metabolism","author":"Ecarot\u2010Charrier B.","year":"1977"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-87152-8_2"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(80)90072-6"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.12.6406"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/259132a0"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(74)90543-6"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.2.901"},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"1617","DOI":"10.4049\/jimmunol.115.6.1617","article-title":"Rapid isolation of antigens from cells with a staphylococcal protein A\u2010antibody adsorbent: parameters of the interaction of antibody\u2010antigen complexes with protein A","volume":"115","author":"Kessler S. 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