{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T00:34:30Z","timestamp":1699835670958},"reference-count":44,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,7,20]],"date-time":"2006-07-20T00:00:00Z","timestamp":1153353600000},"content-version":"vor","delay-in-days":8952,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1982,1,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The cytotoxic response of human lymphocytes to the autologous lymphoblastoid cell line (LCL) was investigated with five Epstein\u2010Barr virus (EBV)\u2010seropositive and four seronegative donors and an additional donor who seroconverted during the course of the study. LCL stimulation was carried out usually at a stimulator\u2010to\u2010responder cell ratio which varied from 1:200 to 1:20,000. Cytotoxic T cells from 7\u2010 and 14\u2010day cultures were assayed on autologous and allogeneic LCL target cells and the K562 line, using the <jats:sup>51<\/jats:sup>Cr release assay. Without exception, the cytotoxic T cells generated in 7\u2010 and 14\u2010day LCL\u2010stimulated cultures from seropositive donors were strongly lytic for the autologous LCL. With some donors, the cytotoxic response increased with increasing autologous LCL stimulation, while with others even the lowest level of stimulation induced a potent response. Overall, the cytotoxic T cells generated in cultures from seropositive donors showed a striking preferential lysis of the autologous LCL and cross\u2010reactive killing of allogeneic LCL target cells matched for certain HLA\u2010B antigens. Lysis of the K562 line, an indicator target for natural\u2010killer\u2010like activity, was consistently negligible. In comparison to the considerable self\u2010preferred lysis obtained with T cells from LCL\u2010stimulated cultures, the level of autologous killing by T cells generated in control unstimulated cultures was relatively low. The cytotoxicity patterns obtained with cytotoxic T cells generated in cultures from seronegative donors were markedly different from those obtained with seropositive donors. The level of autologous killing by T cells harvested from LCL\u2010stimulated cultures was considerably lower than that obtained with seropositive donors and often did not increase above that seen in control cultures, except at the highest stimulatory dose. Moreover, preferential lysis of the autologous LCL target cells by T cells from LCL\u2010stimulated cultures was essentially due to the foetal calf serum (FCS)\u2010induced cytotoxicity and there was no obvious HLA\u2010antigen\u2010restricted killing of allogeneic target cells. Lysis of K562 target cells was generally more pronounced at the highest level of stimulation than that observed with seropositive donors. The distinctive cytotoxic T\u2010cell response patterns observed with the seropositive and seronegative donor cultures were both observed with cultures from an individual donor who was tested before and after infectious mononucleosis. Our cytotoxicity data are interpreted as indicating that with LCL\u2010stimulated cultures from seropositive donors the cytotoxic T\u2010cell response to autologous LCL is predominantly directed against EBV\u2010associated antigens and is HLA\u2010antigen restricted. In contrast, with seronegative donor cultures, the cytotoxic T\u2010cell response is primarily due to FCS\u2010induced cytotoxicity, although a response against other LCL\u2010associated antigens may be superimposed in cultures stimulated with high levels of autologous LCL.<\/jats:p>","DOI":"10.1002\/ijc.2910290108","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T18:45:53Z","timestamp":1171910753000},"page":"41-48","source":"Crossref","is-referenced-by-count":10,"title":["Generation <i>in vitro<\/i> of HLA\u2010restricted EB virus\u2010specific cytotoxic human T cells by autologous lymphoblastoid cell lines: The roles of previous EB virus infection and foetal calf serum"],"prefix":"10.1002","volume":"29","author":[{"given":"I. S.","family":"Misko","sequence":"first","affiliation":[]},{"given":"R. G.","family":"Kane","sequence":"additional","affiliation":[]},{"given":"J. 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