{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T22:21:46Z","timestamp":1757542906766},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":5183,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1992,5,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The neural\u2010cell\u2010adhesion molecule (NCAM) is expressed in all small\u2010cell lung cancers (SCLC) and in approximately 20% of non\u2010small\u2010cell lung tumors (non\u2010SCLC). These NCAM\u2010positive lung tumors have a poor prognosis compared with NCAM\u2010negative tumors. Multiple NCAM protein isoforms are expressed from a single\u2010copy gene as a result of alternative splicing and\/or post\u2010translational modifications. Therefore, we studied the NCAM isoforms expressed in a human small\u2010cell lung\u2010cancer cell line, H69. NCAM mRNA transcripts of 7.2, 6.7, 4.3 and 4.0 were detected in these cells on Northern blots. Since the various NCAM isoforms may have different biological properties, we performed a more precise examination of NCAM mRNAs, using polymerase chain reactions (PCR) with primers flanking the various NCAM exon boundaries. The shortest alternatively spliced sequence that we found was the trinucleotide AAG located between exon 12 and 13 in the so\u2010called hinge region of the NCAM protein. This AAG trinucleotide was present in the majority of the NCAM mRNAs. A second alternatively spliced 30 nt\u2010exon VASE (immunoglobulinvariable domain\u2010like alternatively spliced exon) was present in all NCAM transcript isoforms at the exon 7\/exon 8 junction. VASE resulted in the insertion of 10 amino acids into the 4th immunoglobulin\u2010like loop of the NCAM protein. Within the limits of the PCR methodology, no evidence for the presence of mRNA containing exon 15, encoding the glycosyl\u2010phosphatidylinositol\u2010linked (GPI\u2010linked) NCAM isoform in H69 cells was obtained. Considering that H69 cells express 2 major NCAM protein classes (NCAM\u2010180 and NCAM\u2010140), and that the VASE and AAG alternative mRNA splice variants result in minor differences in protein sizes, at least 8 polypeptide isoforms of NCAM might be expressed in H69 cells that contribute to the binding interactions of NCAM.<\/jats:p>","DOI":"10.1002\/ijc.2910510212","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T22:58:44Z","timestamp":1171925924000},"page":"238-243","source":"Crossref","is-referenced-by-count":21,"title":["Alternative splicing of neural\u2010cell\u2010adhesion molecule mRNA in human small\u2010cell lung\u2010cancer cell line H69"],"prefix":"10.1002","volume":"51","author":[{"given":"C. E. C.","family":"Moolenaar","sequence":"first","affiliation":[]},{"given":"Cynthia","family":"Pieneman","sequence":"additional","affiliation":[]},{"given":"Frank S.","family":"Walsh","sequence":"additional","affiliation":[]},{"given":"Wolter J.","family":"Mooi","sequence":"additional","affiliation":[]},{"given":"Rob J. A. M.","family":"Michalides","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.114.1.143"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.10.5.2012"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(90)81420-S"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910470122"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1242\/dev.104.1.165","article-title":"Complete sequence and in vitro expression of a tissue\u2010specific phosphatidylinositollinked N\u2010CAM isoform from skeletal muscle","volume":"104","author":"Barton C. 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