{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T15:27:40Z","timestamp":1770823660788,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,7,18]],"date-time":"2006-07-18T00:00:00Z","timestamp":1153180800000},"content-version":"vor","delay-in-days":5101,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1992,7,30]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The presence of human papillomavirus (HPV) DNA was assessed in biopsies of tonsillar carcinomas (n = 10) and cases of tonsillitis (n = 7), serving as controls, by general\u2010primer\u2010mediated PCR (GP\u2010PCR). All carcinomas appeared HPV\u2010positive, whereas all cases of tonsillitis were HPV\u2010negative. Additional type\u2010specific PCR for HPV 6, 11, 16, 18, 31 and 33 revealed that 4 carcinomas contained HPV 16 DNA, 4 contained HPV 33 DNA and I contained an HPV 16\/33 double infection. False positivity was excluded by additional Southern blot analysis of type\u2010specific PCR\u2010positive samples (n = 4). Further characterization of GP\u2010PCR products by sequence analysis revealed that 2 carcinomas contained still uncharacter\u2010ized HPV genotypes; one of these also contained HPV 33 DNA and one was negative by type\u2010specific PCR. Application of RNA PCR revealed expression of HPV 16 or HPV 33 E7 encoding spliced E6*l transcripts in all tonsillar carcinomas (n = 4) examined. Additional non\u2010radioactive RNA <jats:italic>in situ<\/jats:italic> hybridization performed on 3 biopsies revealed the presence of HPV 16 or HPV 33 E7 transcripts exclusively localized within the carcinoma cells, whereas stroma stained negative. These findings strongly support a role for certain HPV types in the pathogenesis of tonsillar carcinomas.<\/jats:p>","DOI":"10.1002\/ijc.2910510602","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T22:55:19Z","timestamp":1171925719000},"page":"845-850","source":"Crossref","is-referenced-by-count":124,"title":["Prevalence and expression of human papillomavirus in tonsillar carcinomas, indicating a possible viral etiology"],"prefix":"10.1002","volume":"51","author":[{"given":"Peter J. F.","family":"Snijders","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frans V.","family":"Cromme","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriaan J. C. Den","family":"Van Brule","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henri F. 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