{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T18:24:52Z","timestamp":1775240692589,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":3897,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1995,11,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Thde t( 11;22)(q24;q 12) and t(21;22)(q22;q 12) are specific chromosomal translocations found in the Ewinhd family of tumors including ES, PNET and Askin tumors. In these translocations, the amino\u2010terminal portion of the <jats:italic>EWS<\/jats:italic> gene located in 22q 12 fuses to the carboxyl\u2010terminal portion of the <jats:italic>FLI\u2010I<\/jats:italic> gene located in 11 q24 or the ERG gene located in 21 q22, which belong to the ets oncogene super\u2010family of transcription activators. We investigated the chimeric mRNAs of 15 ESs (7 cell lines and 8 tumor samples) and 7 PNETs (3 cell lines and 4 tumor samples) using the RT\u2010PCR method and sequencing. We detected 2 types of <jats:italic>EWS\u2010ERG<\/jats:italic> chimeric mRNA in 2 ES cell lines and I PNET tumor sample in addition to 4 types of <jats:italic>EWS\u2010FU\u20101<\/jats:italic> chimeric mRNA in 11 ESs (4 cell lines and 7 tumor samples) and 4 PNETs (2 cell lines and 2 tumor samples). There seemed to be no association between the type of chimeric mRNA and clinical features such as sex, age, primary site and histopathology of the patients. All of the chimeric mRNAs are generated from in\u2010frame junctions and are thought to encode fusion proteins that may be the molecular mechanism involved in the Ewing family of tumors.<\/jats:p>","DOI":"10.1002\/ijc.2910630407","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T01:11:41Z","timestamp":1171933901000},"page":"500-504","source":"Crossref","is-referenced-by-count":45,"title":["<i>EWS\u2010FLI\u20101<\/i> and <i>EWS\u2010ERG<\/i> chimeric mRNAs in Ewing's sarcoma and primitive neuroectodermal tumor"],"prefix":"10.1002","volume":"63","author":[{"given":"Kohmei","family":"Ida","sequence":"first","affiliation":[]},{"given":"Shigetoshi","family":"Kobayashi","sequence":"additional","affiliation":[]},{"given":"Tomohiko","family":"Taki","sequence":"additional","affiliation":[]},{"given":"Ryoji","family":"Hanada","sequence":"additional","affiliation":[]},{"given":"Fumio","family":"Bessho","sequence":"additional","affiliation":[]},{"given":"Shunji","family":"Yamamori","sequence":"additional","affiliation":[]},{"given":"Tohru","family":"Sugimoto","sequence":"additional","affiliation":[]},{"given":"Misao","family":"Ohki","sequence":"additional","affiliation":[]},{"given":"Yasuhide","family":"Hayashi","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198308253090817"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.5.6.908"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/363640a0"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199408043310503"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/359162a0"},{"key":"e_1_2_1_7_1","first-page":"1294","article-title":"Detection of the (11;22)(q24;q12) translocation of Ewing's sarcoma and peripheral neuroectodermal tumor by reverse transcription polymerase chain reaction","volume":"143","author":"Downing J. R.","year":"1993","journal-title":"Amer. J. Pathol."},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19890701)64:1<43::AID-CNCR2820640109>3.0.CO;2-F"},{"key":"e_1_2_1_9_1","first-page":"17","article-title":"Establishment and characterization of the cell lines derived from malignant tumors in children","volume":"8","author":"Hayashi Y.","year":"1991","journal-title":"J. Saitama Child. Med."},{"key":"e_1_2_1_10_1","first-page":"795","volume-title":"Principles and practice of paediatric oncology","author":"Horowitz M. E.","year":"1993"},{"key":"e_1_2_1_11_1","first-page":"2865","article-title":"An RNA\u2010binding protein gene, TLS\/FUS, is fused to ERG in human myeloid leukemia with t(16;21) chromosomal translocation","volume":"54","author":"Ichikawa H.","year":"1994","journal-title":"Cancer Res."},{"key":"e_1_2_1_12_1","first-page":"5284","article-title":"Mutations of the p53 gene are involved in Ewing's sarcoma but not in neuroblastomas","volume":"53","author":"Komuro H.","year":"1993","journal-title":"Cancer Res."},{"key":"e_1_2_1_13_1","first-page":"2837","article-title":"Fusion of the EWS and WT1 genes in the desmoplastic small round cell tumor","volume":"54","author":"Ladanyi M.","year":"1994","journal-title":"Cancer Res."},{"key":"e_1_2_1_14_1","first-page":"183","article-title":"Neuroectodermal associated antigens on Ewing's sarcoma cell lines","volume":"47","author":"Lipinski M.","year":"1987","journal-title":"Cancer Res."},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.12.5752"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.13.12.7393"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-200X.1991.tb02566.x"},{"key":"e_1_2_1_18_1","first-page":"5833","article-title":"Structure and expression of human Fli\u20101 gene","volume":"52","author":"Prasad D. D. K.","year":"1992","journal-title":"Cancer Res."},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.17.6131"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.21.10280"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0294-146"},{"key":"e_1_2_1_22_1","first-page":"1","volume-title":"Clinical pediatric oncology","author":"Sutow W. W.","year":"1977"},{"key":"e_1_2_1_23_1","first-page":"497","article-title":"Chromosomal translocation in Ewing's sarcoma","volume":"309","author":"Turc\u2010Carel C.","year":"1983","journal-title":"New Engl. J. Med."},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1994.419"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0893-341"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1993.tb06137.x"}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fijc.2910630407","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.2910630407","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T02:48:43Z","timestamp":1698288523000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.2910630407"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,11,15]]},"references-count":25,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1995,11,15]]}},"alternative-id":["10.1002\/ijc.2910630407"],"URL":"https:\/\/doi.org\/10.1002\/ijc.2910630407","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,11,15]]}}}