{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T17:10:33Z","timestamp":1760980233410},"reference-count":26,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":10841,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1975,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>C<jats:sub>57<\/jats:sub>BL bone marrow cells were separated on the basis of their sedimentation velocity at unit gravity and cell fractions cultured in agar using three types of colony stimulating factor (CSF). Colony\u2010forming cells separated as a single peak (s = 4.4 mm\/hr) in cultures stimulated by mouse lung conditioned medium (CSF<jats:sub>MLCM<\/jats:sub>) or endotoxin serum (CSF<jats:sub>ES<\/jats:sub>). Clusterforming cells were separable into two peaks and the majority were larger than colony\u2010forming cells (s = 5.7 mm\/hr). Partial segregation of colony\u2010forming cells was observed according to the morphological types of colonies generated, large cells tending to generate macrophage colonies and small cells, granulocytic colonies. Large colony\u2010forming cells were more responsive to stimulation by CSF than small cells. Human urine (CSF<jats:sub>HU<\/jats:sub>) appeared unable to stimulate the proliferation of most small colony\u2010forming cells.<\/jats:p><jats:p>Colony\u2010forming cells appear to be a highly heterogeneous population with intrinsic differences in responsiveness to CSF and with differing capacities to generate colonies whose cells differentiate to granulocytes of macrophages.<\/jats:p>","DOI":"10.1002\/jcp.1040850317","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T17:54:26Z","timestamp":1109440466000},"page":"643-653","source":"Crossref","is-referenced-by-count":78,"title":["Heterogeneity of in vitro colony\u2010 and cluster\u2010forming cells in the mouse marrow: Segregation by velocity sedimentation"],"prefix":"10.1002","volume":"85","author":[{"given":"D.","family":"Metcalf","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. 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