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The addition of hEGF to HF cells growing in a medium containing 10% calf serum resulted in a 4\u2010fold increase in the final density. The presence of hEGF also promoted the growth of HF cells in media containing either 1% calf serum or 10% gamma globulin\u2010free serum. The addition of hEGF to quiescent confluent monolayers of HF cells, maintained in a medium with 1% calf serum for 48 hours, resulted in a 10\u2010 to 20\u2010fold increase in the amount of <jats:sup>3<\/jats:sup>H\u2010thymidine incorporation after 20\u201324 hours. The stimulation of thymidine incorporation was maximal at an hEGF concentration of 2 ng\/ml, was dependent on the presence of serum, and was enhanced by the addition of ascorbic acid. In confluent cultures of HF cells, subject to density dependent inhibition of growth, hEGF was able to stimulate DNA synthesis more effectively than fresh calf serum. Human EGF stimulated DNA synthesis in quiescent cultures, however, regardless of cell density. The addition of rabbit anti\u2010hEGF inhibited all effects of this growth factor on HF cells.<\/jats:p>","DOI":"10.1002\/jcp.1040880212","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T02:57:13Z","timestamp":1109386633000},"page":"227-237","source":"Crossref","is-referenced-by-count":282,"title":["Human epidermal growth factor and the proliferation of human fibroblasts"],"prefix":"10.1002","volume":"88","author":[{"given":"Graham","family":"Carpenter","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stanley","family":"Cohen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"91","volume-title":"Advances in Experimental Medicine and Biology","author":"Absher P. 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