{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T11:20:32Z","timestamp":1758280832644},"reference-count":17,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":10292,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1976,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To study the molecular basis of changes in sugar uptake rate in cultured mouse fibroblasts with different physiological states, we have measured the high affinity binding of [<jats:sup>3<\/jats:sup>H] cytochalasin B, a potent sugar transport inhibitor, to actively growing and contact inhibited Balb\/3T3 cells as well as to 3T12 and SV3T3 cells. Binding was the same whether the cells were detached from dishes with EDTA or trypsin. The amount of drug bound to intact cells measured with a centrifugation assay was essentially the same as that bound to cell sonicates measured with equilibrium dialysis. Cytochalasin B binding to intact cells was extremely rapid and reversible over a wide range of drug concentrations, and was not affected by 0.1 M D\u2010glucose or L\u2010glucose in the assay medium. Actively growing and contact inhibited 3T3 cells had a similar number of high affinity cytochalasin B binding sites per cell, while 3T12 and SV3T3 cells had one third to one fourth the number of sites per cell. However, the number of sites per \u03bcg cellular protein appeared to be similar for cells in all of the physiological states examined.<\/jats:p>","DOI":"10.1002\/jcp.1040890436","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T17:28:41Z","timestamp":1109438921000},"page":"751-756","source":"Crossref","is-referenced-by-count":15,"title":["High\u2010affinity cytochalasin b binding to normal and transformed BALB\/3T3 cells"],"prefix":"10.1002","volume":"89","author":[{"given":"Susan J.","family":"Atlas","sequence":"first","affiliation":[]},{"given":"Shin","family":"Lin","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1040720208"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.70.8.2374"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1040850103"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(74)90256-6"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.56.2.487"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(72)90094-2"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1016\/S0021-9258(18)63321-6","article-title":"Evidence for a functional change in the plasma membrane of murine sarcoma virus\u2010infected mouse embryo cells","volume":"245","author":"Hatanaka M.","year":"1970","journal-title":"J. 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