{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T20:46:46Z","timestamp":1771879606244,"version":"3.50.1"},"reference-count":12,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":9837,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1978,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Transformation of chicken embryo fibroblasts with Rous sarcoma virus results in cells with an enhanced rate of hexose uptake. We have examined transport of the glucose analogs 2\u2010deoxyglucose and 3\u20100\u2010methylglucose in cells infected with a temperature sensitive variant of the virus. In cells shifted from restrictive to permissive conditions for transformation, increased transport of the non\u2010phosphorylatable analog 3\u20100\u2010methylglucose occurs at the same time as that of 2\u2010deoxyglucose, a phosphorylatable analog. This enhanced rate of transport can be observed within three hours of the temperature shift. There is a corresponding decrease in the transport rate of both analogs following shift to the restrictive temperature. These results suggest that increased transport is likely to be the primary event in causing transformation\u2010specific changes in sugar metabolism. We have also examined uptake into the internal pools of both the phosphorylated and non\u2010phosphorylated forms of 2\u2010deoxyglucose in normal cells and in cells transformed by the wild\u2010type virus. These data indicate a corresponding increase in the rate of accumulation of the free sugar in transformed cells and point to transport as the rate limiting step in the accumulation of 2\u2010deoxyglucose in both normal and transformed chicken embryo cells.<\/jats:p>","DOI":"10.1002\/jcp.1040940309","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T02:18:33Z","timestamp":1109384313000},"page":"315-319","source":"Crossref","is-referenced-by-count":14,"title":["Increased membrane transport of 2\u2010deoxyglucose and 3\u20100\u2010methylglucose is an early event in the transformation of chick embryo fibroblasts by rous sarcoma virus"],"prefix":"10.1002","volume":"94","author":[{"given":"Dennis R.","family":"Lang","sequence":"first","affiliation":[]},{"given":"Michael J.","family":"Weber","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1040890430"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1040850103"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(74)90256-6"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/newbio246181a0"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(70)90429-0"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(71)90047-X"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.68.11.2739"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1040830205"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1040890434"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.71.10.4129"},{"key":"e_1_2_1_12_1","volume-title":"The Metabolism of Tumors","author":"Warburg O.","year":"1930"},{"key":"e_1_2_1_13_1","doi-asserted-by":"crossref","first-page":"2978","DOI":"10.1016\/S0021-9258(19)43997-5","article-title":"Hexose transport in normal and Rous sarcoma virus\u2010transformed cells","volume":"248","author":"Weber M. J.","year":"1973","journal-title":"J. Biol. Chem."}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.1040940309","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.1040940309","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,12]],"date-time":"2023-11-12T13:21:22Z","timestamp":1699795282000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.1040940309"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1978,3]]},"references-count":12,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1978,3]]}},"alternative-id":["10.1002\/jcp.1040940309"],"URL":"https:\/\/doi.org\/10.1002\/jcp.1040940309","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[1978,3]]}}}