{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:14:35Z","timestamp":1760188475750},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":8831,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1980,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have compared the subcellular sites of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> and presumably also superoxide\u2010(O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup>) production, and certain aspects of metabolic responses (O<jats:sub>2<\/jats:sub> consumption, O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup> production) of stimulated neutrophils from human blood and those elicited into guinea pig peritonea. Stimulation was accomplished with either opsonized zymosan or phorbol\u201012\u2010myristate\u201013\u2010acetate (PMA). Striking quantitative differences were observed between these cell types with regard to the increased respiration and O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup> production observed during stimulation. These differences were most apparent when opsonized zymosan served as the stimulating agent. They were minimized when the soluble stimulating agent, PMA, was used. With either stimulus, the subcellular sites of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> production were the same for both types of neutrophils, i.e., the plasmalemma and phagosomal membranes. No H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> production could be detected cytochemically in the absence of stimulation.<\/jats:p><jats:p>Treatment of both unstimulated human blood and elicited guinea pig peritoneal neutrophils with the nonpenetrating, covalently linking reagent, p\u2010diazobenzenesulfonic acid, failed to diminish O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup> production upon subsequent stimulation, in contrast to a previous report. These data are discussed in terms of the possible cytological arrangements of the respiratory enzyme(s), and the different modes of stimulation of neutrophil metabolism by various agents. Ancillary data on elicited mouse peritoneal neutrophils are presented.<\/jats:p>","DOI":"10.1002\/jcp.1041050319","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T16:06:50Z","timestamp":1109434010000},"page":"541-551","source":"Crossref","is-referenced-by-count":61,"title":["Comparative aspects of oxidative metabolism of neutrophils from human blood and guinea pig peritonea: Magnitude of the respiratory burst, dependence upon stimulating agents, and localization of the oxidases"],"prefix":"10.1002","volume":"105","author":[{"given":"J. A.","family":"Badwey","sequence":"first","affiliation":[]},{"given":"J. T.","family":"Curnutte","sequence":"additional","affiliation":[]},{"given":"J. M.","family":"Robinson","sequence":"additional","affiliation":[]},{"given":"J. K.","family":"Lazdins","sequence":"additional","affiliation":[]},{"given":"R. T.","family":"Briggs","sequence":"additional","affiliation":[]},{"given":"M. J.","family":"Karnovsky","sequence":"additional","affiliation":[]},{"given":"M. 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