{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:18:48Z","timestamp":1762100328380},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":8557,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1981,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Trifluoperazine (TFP) blocks spreading and migration of cultured mammalian cells. These are calcium\u2010dependent and microfilament\u2010mediated processes. Calmodulin, a regulator of many calcium\u2010dependent processes in cells, is selectively inhibited by TFP. Cell spreading on a plastic\u2010 or collagen\u2010coated substratum was reversibly inhibited by 10 \u03bcM TFP. The drug blocks cell spreading even in the presence of 1 mM cAMP. TFP is as effective as cytochalasin B (CB), an inhibitor of microfilament function, in blocking cell spreading. All cell lines tested, whether \u201cnormal\u201d or virally transformed, failed to spread in TFP. The drug, at a concentration sufficient to inhibit spreading, does not interfere with the initial attachment of a cell to a plastic surface. Cells plated in the presence of 10 \u03bcM TFP attach at a rate and to an extent equal to untreated controls. TFP added to already spread cells results in a reversible cell rounding. Detection of fibronectin by indirect immunofluorescence suggests TFP\u2010induced cell rounding is not due to shedding of fibronectin from the cell surface. TFP reversibly blocks cell migration into a wound edge almost as effectively as CB. We suggest that TFP interferes with these microfilament\u2010mediated functions by direct action on the microfilaments or indirect action by inactivating calmodulin.<\/jats:p>","DOI":"10.1002\/jcp.1041080303","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T15:50:27Z","timestamp":1109433027000},"page":"299-307","source":"Crossref","is-referenced-by-count":29,"title":["Trifluoperazine inhibits spreading and migration of cells in culture"],"prefix":"10.1002","volume":"108","author":[{"given":"Charles G.","family":"Connor","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard C.","family":"Brady","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara L.","family":"Brownstein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.73.10.3603"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.493982"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(80)90849-9"},{"key":"e_1_2_1_5_1","first-page":"16a","article-title":"The effect of calmodulin inhibitors on optic cup formation","volume":"87","author":"Brady R. 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