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Endothelial cell tropoelastin cross\u2010reacts with antiserum to bovine ligamentum nuchae elastin and comigrates on SDS\u2010PAGE with tropoelastins from fetal bovine ligamentum nuchae fibroblasts, aortic smooth muscle cells, and ear chondroblasts at an apparent molecular weight of 70,000. Endothelial cells synthesize only one\u2010third as much elastin as these other cell types, however. Approximately 80% of the elastin synthesized by endothelial cells in confluent culture is released into the culture medium. The remaining 20% remains associated with the cell layer and is readily extractable with dilute acetic acid as un\u2010cross\u2010linked, 70,000\u2010dalton tropoelastin. The addition of \u03b2\u2010aminopro\u2010pronitrile to culture medium did not alter the ratio of tropoelastin in the medium and cell layer, suggesting that cross\u2010linking of tropoelastin does not occur in culture.<\/jats:p><jats:p>Immunofluorescent staining of confluent endothelial cell cultures with antielastin serum demonstrated elastin occurring as a web\u2010like network of fine filaments extending throughout the extracellular space. The fibrous elastin was different in organization and distribution from fibers stained with antifibronectin serum, which were localized primarily beneath the cell layer and in regions of cell\u2010cell contact. Extracellular matrix remaining after solu\u2010bilization of cellular material with Triton X\u2010100 stained positive for fibronectin, but not for elastin.<\/jats:p>","DOI":"10.1002\/jcp.1041160304","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T14:31:18Z","timestamp":1109428278000},"page":"282-288","source":"Crossref","is-referenced-by-count":72,"title":["Elastin production by cultured calf pulmonary artery endothelial cells"],"prefix":"10.1002","volume":"116","author":[{"given":"Robert P.","family":"Mecham","sequence":"first","affiliation":[]},{"given":"Judy","family":"Madaras","sequence":"additional","affiliation":[]},{"given":"John A.","family":"McDonald","sequence":"additional","affiliation":[]},{"given":"Una","family":"Ryan","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.7.3273"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1974.tb03599.x"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(75)90067-7"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(80)80050-7"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(79)91190-2"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.90.2.372"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(83)90261-8"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(80)90575-8"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.85.2.467"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041070203"},{"key":"e_1_2_1_12_1","first-page":"109","article-title":"Control of cellular proliferation by the fibroblast and epidermal growth factors","volume":"48","author":"Gospodarowicz D.","year":"1978","journal-title":"Natl. 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