{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,12]],"date-time":"2023-11-12T14:40:31Z","timestamp":1699800031085},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,5,9]],"date-time":"2005-05-09T00:00:00Z","timestamp":1115596800000},"content-version":"vor","delay-in-days":12335,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Exp. Zool."],"published-print":{"date-parts":[[1971,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Actomyosin from crayfish skeletal muscle will superprecipitate in the presence of ATP without added Ca<jats:sup>++<\/jats:sup> or Mg<jats:sup>++<\/jats:sup>. EDTA totally inhibits Ca<jats:sup>++<\/jats:sup>\u2010induced superprecipitation of actomyosin, but in some cases Mg<jats:sup>++<\/jats:sup> will reverse this effect. It is suggested that Mg<jats:sup>++<\/jats:sup> and not Ca<jats:sup>++<\/jats:sup> is a prerequisite for superprecipitation with ATP alone. EDTA only partly inhibits heavy metal\u2010induced actomyosin superprecipitation.<\/jats:p><jats:p>Heavy metal cations induce far greater actomyosin superprecipitation than Ca<jats:sup>++<\/jats:sup>, and they can even enhance previously formed Ca<jats:sup>++<\/jats:sup>\u2010induced precipitates. It is suggested that heavy metal cations may be more strongly bound to crayfish actomyosin than Ca<jats:sup>++<\/jats:sup>, and that they may displace Ca<jats:sup>++<\/jats:sup> from existing actomyosin binding sites. Heavy metal potentiation of muscular contraction may thus involve direct action at myofibril binding sites.<\/jats:p><jats:p>Caffeine and quinine are without effect either on the rate or extent of development of actomyosin precipitates in the presence of ATP, ATP plus Ca<jats:sup>++<\/jats:sup>, and ATP plus Zn<jats:sup>++<\/jats:sup>. These observations rule out any direct effect of these drugs at the myofibril level during drug\u2010induced potentiation of muscular contraction.<\/jats:p>","DOI":"10.1002\/jez.1401770403","type":"journal-article","created":{"date-parts":[[2005,6,11]],"date-time":"2005-06-11T14:20:54Z","timestamp":1118499654000},"page":"407-415","source":"Crossref","is-referenced-by-count":1,"title":["Superprecipitation of actomyosin extracted from crayfish skeletal muscle: The effect of heavy metal cations, EDTA and drugs"],"prefix":"10.1002","volume":"177","author":[{"given":"Henry","family":"Huddart","sequence":"first","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,5,9]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1093\/icb\/7.3.647","article-title":"The role of cell calcium in the contraction of single cannulated muscle fibres","volume":"7","author":"Ashley C. C.","year":"1967","journal-title":"Am. 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