{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T08:24:58Z","timestamp":1768551898944,"version":"3.49.0"},"reference-count":15,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,12,8]],"date-time":"2005-12-08T00:00:00Z","timestamp":1134000000000},"content-version":"vor","delay-in-days":8012,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[1984,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The effects of 6\u2010diazo\u20105\u2010oxo\u2010L\u2010norleucine (DON), 2\u2010deoxy\u2010D\u2010glucose (DOG), and tunicamycin (TM) on the replication of poliovirus (PV) and vesicular stomatitis virus (VSV) were examined. During a 48\u2010hr replication period, TM, DON, and DOG inhibit VSV plaque formation in HEp\u20102 cells by 99.9%, 99.8%, and 99.9% respectively. Inhibition of VSV by DON is reversed with glutamine. Although all three agents are known to affect glycoprotein synthesis, DON and DOG also inhibit plaque formation of viruses devoid of structural glycoproteins. Thus, plaque formation of PV types 1 and 3 and Coxsackie B3 virus is delayed in HEp\u20102 and Buffalo green monkey kidney cells during exposure to these agents. Since these viruses do not contain glycoproteins and since concentrations up to 10 \u03bcg TM\/ml cause no significant inhibition of PV, DON and DOG are affecting another viral or cellular process. Inhibition of PV replication by DON is reversed by addition of 25 mM glutamine or marginally by exposure to a combination of 5 mM concentrations of cytidine, uridine, adenosine monophosphate, and guanosine monophosphate. Inhibition of PV replication by DOG is reversed with 5 mM uridine alone. During DON exposure of HEp\u20102 cells infected with PV, the amount of <jats:sup>3<\/jats:sup>H\u2010uridine incorporation at 5.5 hr postinfection (pi) is reduced to 53% of untreated controls, an amount 11% greater than incorporation in cultures infected with PV but not treated with DON. These data indicate that the inhibition of PV replication by DON or DOG occurs at the level of viral RNA synthesis, while the primary target of these agents during VSV replication is probably glycosylation.<\/jats:p>","DOI":"10.1002\/jmv.1890140210","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T11:58:30Z","timestamp":1172836710000},"page":"159-167","source":"Crossref","is-referenced-by-count":10,"title":["Poliovirus and vesicular stomatitis virus replication in the presence of 6\u2010diazo\u20105\u2010oxo\u2010L\u2010norleucine or 2\u2010deoxy\u2010D\u2010glucose"],"prefix":"10.1002","volume":"14","author":[{"given":"Gerald","family":"Goldstein","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Louis E.","family":"Guskey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,8]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(73)90340-1"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1128\/aem.44.2.317-320.1982","article-title":"Development of a shaker culture of Buffalo green monkey kidney cells and its potential use for detection of enteroviruses","volume":"44","author":"Goldstein G","year":"1982","journal-title":"Applied and Environmental Microbiology"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0380-1330(83)71920-9"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-54-1-115"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-33-3-389"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(73)90127-X"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"3933","DOI":"10.1016\/S0021-9258(17)34780-4","article-title":"Human placental amidophosphoribosyl\u2010transferase comparison of the kinetics of glutamine and ammonia utilization","volume":"253","author":"King G","year":"1978","journal-title":"Journal of Biological Chemistry"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(72)90529-6"},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1128\/jvi.21.1.375-385.1977","article-title":"Tunicamycin inhibits glycosylation and multiplication of Sindbis and vesicular stomatitis viruses","volume":"21","author":"Leavitt R","year":"1977","journal-title":"Journal of Virology"},{"key":"e_1_2_1_11_1","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1128\/jvi.28.1.368-374.1978","article-title":"Assembly of viral membranes: maturation of the vesicular stomatitis virus glycoprotein in the presence of tunicamycin","volume":"28","author":"Morrison TG","year":"1978","journal-title":"Journal of Virology"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-662-38439-8_39"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(76)90292-X"},{"key":"e_1_2_1_14_1","first-page":"210","article-title":"Inhibition of vesicular stomatitis virus multiplication in Vero cells by 2\u2013deoxy\u2010D\u2010glucose","volume":"23","author":"Sabina LR","year":"1979","journal-title":"Acta Virologica"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1974.tb03828.x"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-66101-3_4"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.1890140210","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.1890140210","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T18:50:14Z","timestamp":1697827814000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.1890140210"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1984,1]]},"references-count":15,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1984,1]]}},"alternative-id":["10.1002\/jmv.1890140210"],"URL":"https:\/\/doi.org\/10.1002\/jmv.1890140210","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[1984,1]]}}}