{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T10:09:45Z","timestamp":1773396585994,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,10,11]],"date-time":"2004-10-11T00:00:00Z","timestamp":1097452800000},"content-version":"vor","delay-in-days":4270,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[1993,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The major constituent of the paired helical filaments (PHFs) of Alzheimer's disease is the abnormally phosphorylated form of the microtubule\u2010associated protein, \u03c4. Monoclonal antibody (mAb) Tau\u20101 is used extensively to stain normal human \u03c4, and \u03c4 isolated from the brains of Alzheimer's disease patients after dephosphorylation. We used a panel of 6 synthetic peptides to localize the minimal epitope of Tau\u20101 between amino acids 192\u2013204. All 4 serine residues within this fragment were later phosphorylated individually by chemical methods, and it was shown that none of the peptides carrying a single phosphate group were recognized by the antibody. The serines included those that are probably not transformed in AD and consequently, conclusions drawn about malfunctioning kinase activity, based on Tau\u20101 immunoreactivity, can be extremely misleading. The recognition was restored at a decreased level when one of the serines was replaced by an alanine residue. mAb AT8 was made by immunizing with the PHFs and was reported to recognize the same region of the protein in a phosphorylated form. AT8 did not, however, cross\u2010react with any of the singly phosphorylated peptides, indicating that the recognition site of the two antibodies are not entirely complementary or the binding to AT8 needs multiple phosphorylation of the antigen. The abolished recognition of the phosphorylated peptides cannot be attributed to a conformational change due to phosphorylation, since all peptides exhibited reverse\u2010turn secondary structures, as indicated by circular dichroism (CD) spectroscopy. Ant\u2010\u03c4 mAbs may distinguish between phosphorylated and non\u2010phosphorylated forms of epitopes regardless of the location of the phosphate group. \u00a9 1993 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.490340212","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T18:40:30Z","timestamp":1117305630000},"page":"243-249","source":"Crossref","is-referenced-by-count":164,"title":["Recognition of the minimal epitope of monoclonal antibody Tau\u20101 depends upon the presence of a phosphate group but not its location"],"prefix":"10.1002","volume":"34","author":[{"given":"G. I.","family":"Szendrei","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V. M.\u2010Y.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"L.","family":"Otvos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,11]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830191033"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(78)80795-9"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","unstructured":"AthertonE SheppardRC(1985):Solid phase peptide synthesis using N\u03b1fluorenylmethoxycarbonylamino acid pentafluorophenyl esters. J Chem Soc Chem Comm165\u2013166.","DOI":"10.1039\/C39850000165"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(89)91396-6"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"17577","DOI":"10.1016\/S0021-9258(18)45420-8","article-title":"Phosphorylation of tau proteins to a state like in Alzheimer's brain is catalyzed by a calcium\/calmodulindependent kinase and modulated by phospholipids","volume":"262","author":"Baudier J","year":"1987","journal-title":"J Biol Chem"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1992.tb05204.x"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.101.4.1371"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-4039(00)97399-4"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1992.tb05272.x"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(88)90447-0"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00519a032"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(89)90210-9"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.13.4913"},{"key":"e_1_2_1_15_1","first-page":"1352","article-title":"The T cell response to the glycoprotein D of the herpes simplex virus: The significance of antigen conformation","volume":"132","author":"Heber\u2010Katz E","year":"1985","journal-title":"J Immunol"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(92)90983-Q"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(87)80670-1"},{"key":"e_1_2_1_18_1","first-page":"817","article-title":"Epitopes that span the tau molecule are shared with paired helical filaments","volume":"1","author":"Kosik KS","year":"1988","journal-title":"Neurology"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(05)80112-3"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(92)91264-Q"},{"key":"e_1_2_1_21_1","doi-asserted-by":"crossref","first-page":"3992","DOI":"10.1128\/jvi.66.7.3996-4002.1992","article-title":"Posttranslational side chain modification of a viral epitope results in diminished recognition by specific T cells","volume":"66","author":"Larson JK","year":"1992","journal-title":"J Virol"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-011-2264-1_34"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.6.1998"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.19.7384"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1899488"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.12.5384"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(71)90443-X"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01024886"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3011.1989.tb01501.x"},{"key":"e_1_2_1_30_1","first-page":"185","volume-title":"Innovation and Perspectives in Solid\u2010Phase Synthesis and Related Technologies: Peptides, Polypeptides and Oligonucleotides","author":"Otvos L","year":"1992"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(92)85457-5"},{"key":"e_1_2_1_32_1","first-page":"16","volume-title":"The Peptides","author":"Woody RW","year":"1985"}],"container-title":["Journal of Neuroscience Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjnr.490340212","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jnr.490340212","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T18:39:29Z","timestamp":1698172769000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jnr.490340212"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,2]]},"references-count":31,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1993,2]]}},"alternative-id":["10.1002\/jnr.490340212"],"URL":"https:\/\/doi.org\/10.1002\/jnr.490340212","archive":["Portico"],"relation":{},"ISSN":["0360-4012","1097-4547"],"issn-type":[{"value":"0360-4012","type":"print"},{"value":"1097-4547","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,2]]}}}