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By this and the previous improvements (e.g., a new vacuum system with a recorder, cool traps, automatic conversion) much better results were obtained than before. It was even possible to use (in addition to the standard methods, e.g., thin\u2010layer chromatography and amino acid analysis) mass spectrometry without preceding gas chromatography for identification of the released PTH amino acids. Our experience with the various methods, especially mass spectrometry, is described and the techniques are compared. The results obtained by the described methods on the amino acid sequences of the 14 ribosomal proteins are summarized.<\/jats:p>","DOI":"10.1002\/jss.400030505","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T09:50:49Z","timestamp":1117273849000},"page":"426-447","source":"Crossref","is-referenced-by-count":71,"title":["Studies on the primary structure of 14 proteins from the large subunit of escherichia coli ribosomes with an improved protein sequenator and with mass spectrometry"],"prefix":"10.1002","volume":"3","author":[{"given":"B.","family":"Wittmann\u2010Liebold","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. W.","family":"Geissler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Marzinzig","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,2,19]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1515\/bchm2.1973.354.2.1415"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(73)80382-5"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1967.tb00047.x"},{"key":"e_1_2_1_5_2","unstructured":"Graffunder H. 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G. personal communication."},{"key":"e_1_2_1_18_2","series-title":"Cold Spring Harbor Laboratory Monograph Series 615\u2013667","volume-title":"The Ribosome","author":"St\u00f6ffler G.","year":"1974"},{"key":"e_1_2_1_19_2","series-title":"Cold Spring Harbor Laboratory Monograph Series 115\u2013140","volume-title":"The Ribosome","author":"Wittmann H. G.","year":"1974"},{"key":"e_1_2_1_20_2","volume-title":"Atlas of Protein Sequence and Structure","author":"Dayhoff M. 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