{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T22:30:18Z","timestamp":1757543418020},"reference-count":23,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,2,19]],"date-time":"2004-02-19T00:00:00Z","timestamp":1077148800000},"content-version":"vor","delay-in-days":9910,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Supramol Struct"],"published-print":{"date-parts":[[1977,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>As part of a program to investigate the behavior and interactions of glycolipids in biological membranes we have synthesized spin\u2010labeled derivatives of 2 families of carbohydrate\u2010bearing ceramides (glycosphingolipids): simple neutral glycolipids and gangliosides. Galactosyl ceramide has been synthesized with the spin label at 3 different positions on the fatty acid chain. It has been studied in bilayers of various different lipids and lipid mixtures and compared to the corresponding phospholipid spin labels. Considerable similarity has been found between the behavior of galactosyl ceramide and phosphatidylcholine. These similarities include a negligible flip\u2010flop rate, a flexibility gradient in the acyl chains, and exclusion from phosphatidylserine domains in the face of a Ca<jats:sup>2+<\/jats:sup>\u2010induced lateral phase separation. Evidence for dramatic clustering of simple neutral glycolipids has not been found. Glycosphingolipids do seem to have the capacity to increase rigidity in fluid lipid bilayers. A general procedure has been developed for covalent attachment of a nitroxide spin label to the headgroup region of complex glycolipids such as gangliosides. Studies of beef brain gangliosides labeled in this manner and incorporated into bilayers of phosphatidylcholine indicate that the headgroup oligosaccharides are in rapid, random motion as opposed to being in any way immobilized. This headgroup mobility depends very little on the fluidity or rigidity of the bilayer. However, headgroup mobility decreases, perhaps as a result of cooperative headgroup interactions, with increasing bilayer concentration of unlabeled ganglioside.<\/jats:p>","DOI":"10.1002\/jss.400060210","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T00:06:21Z","timestamp":1117238781000},"page":"249-258","source":"Crossref","is-referenced-by-count":31,"title":["Glycosphingolipids in membrane architecture"],"prefix":"10.1002","volume":"6","author":[{"given":"Frances J.","family":"Sharom","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chris W. M.","family":"Grant","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,2,19]]},"reference":[{"key":"e_1_2_1_2_2","unstructured":"McConnellHM:The Neurosciences: Second Study Program: 697 1970."},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00783a003"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja00768a014"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja00768a600"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.69.8.2056"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(75)90196-5"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja00731a005"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(77)91622-9"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0019-2791(74)90118-9"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(76)90319-9"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(76)90382-5"},{"key":"e_1_2_1_13_2","first-page":"301","volume":"28","author":"Radin NS","year":"1972","journal-title":"Methods Enzymol"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(73)90518-4"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00702a008"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00672a026"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja00716a008"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1972.tb54800.x"},{"key":"e_1_2_1_19_2","volume-title":"Spin Labeling, Theory and Applications","author":"McConnell HM","year":"1976"},{"key":"e_1_2_1_20_2","volume-title":"Biology and Chemistry of Eucaryotic Cell Surfaces","author":"Roseman S","year":"1974"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4157(76)90014-9"},{"key":"e_1_2_1_22_2","series-title":"MTP International Review of Science, Biochemistry Series One","volume-title":"Biochemistry of Carbohydrates","author":"Rees DA","year":"1975"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(74)80012-4"},{"key":"e_1_2_1_24_2","doi-asserted-by":"crossref","first-page":"7841","DOI":"10.1016\/S0021-9258(19)42043-7","volume":"249","author":"Ji TH","year":"1974","journal-title":"J Biol Chem"}],"container-title":["Journal of Supramolecular Structure"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjss.400060210","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jss.400060210","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,2]],"date-time":"2023-11-02T02:37:18Z","timestamp":1698892638000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jss.400060210"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1977,1]]},"references-count":23,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1977,1]]}},"alternative-id":["10.1002\/jss.400060210"],"URL":"https:\/\/doi.org\/10.1002\/jss.400060210","archive":["Portico"],"relation":{},"ISSN":["0091-7419","1547-9366"],"issn-type":[{"value":"0091-7419","type":"print"},{"value":"1547-9366","type":"electronic"}],"subject":[],"published":{"date-parts":[[1977,1]]}}}