{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T10:57:20Z","timestamp":1758279440380},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,2,19]],"date-time":"2004-02-19T00:00:00Z","timestamp":1077148800000},"content-version":"vor","delay-in-days":9545,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Supramol Struct"],"published-print":{"date-parts":[[1978,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Using a radioactively tagged, photoaffinity analog of cAMP, 8\u2010azidoadenosine\u20103\u2032,5\u2032\u2010cyclic monophosphate (8\u2010N<jats:sub>3<\/jats:sub> cAMP), and [\u03b3<jats:sup>32<\/jats:sup>P] ATP, the membranebinding properties of both the regulatory and catalytic subunits of the cAMP\u2010activated protein kinase of human erythrocyte membranes were investigated. [<jats:sup>32<\/jats:sup>P] 8\u2010N<jats:sub>3<\/jats:sub> cAMP was used to locate and quantify regulatory subunits. Increased phosphorylation of specific membrane proteins by [\u03b3<jats:sup>32<\/jats:sup>P] ATP was used to determine the presence of the catalytic subunit. The data support a mechanism which operates through a tight membrane\u2010bound regulatory subunit and a catalytic subunit that is released from the membrane when cAMP is present and the Mg \u00b7 ATP concentration is below approximately 10 \u03bcM. The catalytic subunit is not required for the Mg \u00b7 ATP inhibition of 8\u2010N<jats:sub>3<\/jats:sub> cAMP binding. Experiments with a photoaffinity analog of ATP, 8\u2010azidoadenosine triphosphate (8\u2010N<jats:sub>3<\/jats:sub>ATP), support the hypothesis that ATP hydrolysis and phosphorylation are not involved in the regulation. The data indicate that the regulatory subunit contains an ATP regulatory site which inhibits 8\u2010N<jats:sub>3<\/jats:sub> cAMP binding and the release of the catalytic subunit. These results indicate that the membrane\u2010bound type I enzyme (type IM) differs significantly from the soluble (type IS) enzyme studied in other tissues. These enzymes are compartmentalized by being in different cellular locations and are regulated differently by Mg \u00b7 ATP.<\/jats:p>","DOI":"10.1002\/jss.400090107","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T05:24:38Z","timestamp":1117257878000},"page":"57-68","source":"Crossref","is-referenced-by-count":22,"title":["Use of photoaffinity nucleotide analogs to determine the mechanism of ATP regulation of a membrane\u2010bound, cAMP\u2010activated protein kinase"],"prefix":"10.1002","volume":"9","author":[{"given":"James R.","family":"Owens","sequence":"first","affiliation":[]},{"given":"Boyd E.","family":"Haley","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2004,2,19]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)42003-6"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)40885-5"},{"key":"e_1_2_1_4_2","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/S0021-9258(17)32804-1","volume":"252","author":"Hosey MM","year":"1977","journal-title":"J Biol Chem"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)40884-3"},{"key":"e_1_2_1_6_2","doi-asserted-by":"crossref","first-page":"3375","DOI":"10.1016\/S0021-9258(17)33447-6","volume":"251","author":"Rangel\u2010Aldoa R","year":"1975","journal-title":"J Biol Chem"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0026-0495(75)90114-6"},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"7526","DOI":"10.1016\/S0021-9258(17)32882-X","volume":"251","author":"Rangel\u2010Aldao R","year":"1976","journal-title":"J Biol Chem"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(72)90542-6"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.71.9.3580"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00688a018"},{"key":"e_1_2_1_12_2","first-page":"339","volume-title":"Methods in Enzymology","author":"Haley B","year":"1977"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/jss.400050110"},{"key":"e_1_2_1_14_2","unstructured":"OwensJR HaleyB: (Unpublished results)."},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(77)90055-8"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00688a019"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.71.9.3367"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)39985-4"},{"issue":"1","key":"e_1_2_1_19_2","first-page":"151","volume":"7","author":"Owens J","year":"1977","journal-title":"J Supramol Struct"},{"issue":"2","key":"e_1_2_1_20_2","first-page":"117","volume":"8","author":"Owens J","year":"1978","journal-title":"J Supramol Struct"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)44774-1"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)40171-2"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00724a010"},{"key":"e_1_2_1_24_2","doi-asserted-by":"crossref","first-page":"3854","DOI":"10.1016\/S0021-9258(17)40330-9","volume":"252","author":"Corbin J","year":"1976","journal-title":"J Biol Chem"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.68.10.2444"}],"container-title":["Journal of Supramolecular Structure"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjss.400090107","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jss.400090107","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T14:45:22Z","timestamp":1698849922000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jss.400090107"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1978,1]]},"references-count":24,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1978,1]]}},"alternative-id":["10.1002\/jss.400090107"],"URL":"https:\/\/doi.org\/10.1002\/jss.400090107","archive":["Portico"],"relation":{},"ISSN":["0091-7419","1547-9366"],"issn-type":[{"value":"0091-7419","type":"print"},{"value":"1547-9366","type":"electronic"}],"subject":[],"published":{"date-parts":[[1978,1]]}}}