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A basis for this effect was suggested by studies showing that DX also increased the cellular binding of <jats:sup>125<\/jats:sup>I\u2010EGF. DX increased the ability of the cells to bind <jats:sup>125<\/jats:sup>I\u2010EGF only at low physiological concentrations of this polypeptide. Thus, data from <jats:sup>125<\/jats:sup>I\u2010EGF binding to cells incubated without DX produced a linear Scatchard plot, whereas the data from <jats:sup>125<\/jats:sup>I\u2010EGF binding to DX\u2010treated cells led to an upwardly curvilinear Scatchard plot. Measurements of <jats:sup>125<\/jats:sup>I\u2010EGF association with the cell surface and cytoplasm indicated that this binding change involved an alteration of cell surface EGF receptors. The binding change appeared not to involve negatively cooperative interactions between EGF receptors, nor a change in the number of receptors. The binding alteration could be explained by a model in which DX converted 25\u201330% of the cell surface EGF receptors to a form having a fourfold increased affinity.<\/jats:p>","DOI":"10.1002\/jss.400090108","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T05:24:38Z","timestamp":1117257878000},"page":"69-77","source":"Crossref","is-referenced-by-count":24,"title":["Glucocorticoid\u2010mediated alteration in growth factor binding and action: Analysis of the binding change"],"prefix":"10.1002","volume":"9","author":[{"given":"Joffre B.","family":"Baker","sequence":"first","affiliation":[]},{"given":"Dennis D.","family":"Cunningham","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2004,2,19]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(72)90154-4"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/249123a0"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.70.9.2702"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/242399a0"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/262593a0"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.4.1882"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)44568-7"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)83739-0"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.71.1.159"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo-95-3-776"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)41417-8"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1949.tb27297.x"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(67)90297-7"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)42825-1"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(73)80072-5"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/jss.400040211"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.11.5046"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00682a013"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(75)80401-3"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)44186-0"}],"container-title":["Journal of Supramolecular Structure"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjss.400090108","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jss.400090108","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T14:45:23Z","timestamp":1698849923000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jss.400090108"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1978,1]]},"references-count":21,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1978,1]]}},"alternative-id":["10.1002\/jss.400090108"],"URL":"https:\/\/doi.org\/10.1002\/jss.400090108","archive":["Portico"],"relation":{},"ISSN":["0091-7419","1547-9366"],"issn-type":[{"value":"0091-7419","type":"print"},{"value":"1547-9366","type":"electronic"}],"subject":[],"published":{"date-parts":[[1978,1]]}}}