{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T06:25:26Z","timestamp":1779344726949,"version":"3.51.4"},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,10,11]],"date-time":"2004-10-11T00:00:00Z","timestamp":1097452800000},"content-version":"vor","delay-in-days":7834,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Neurobiol."],"published-print":{"date-parts":[[1983,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The temperature\u2010sensitive <jats:italic>Drosophila<\/jats:italic> mutant, <jats:italic>shibire<\/jats:italic><jats:sup>ts1<\/jats:sup> (<jats:italic>shi<\/jats:italic>), has been shown to exhibit a reversible block in synaptic transmission at 30\u00b0C. Various synaptic terminals (neuromuscular, sensory, central) of this mutant were observed by electron microscopy. At 19\u00b0C, all terminals of <jats:italic>shi<\/jats:italic> showed essentially the same structural features as those of wild\u2010type (Oregon\u2010R) flies, while at 30\u00b0C (5 or 10 min of exposure) <jats:italic>shi<\/jats:italic> terminals exhibited various structural changes not seen in the wild type. The major structural change observed in all of the various types of terminals was the accumulation of many pitlike structures on the plasma membrane near presynaptic sites. These structures consisted of a spherical head portion, about 50\u2013100 nm in diameter, and a cylindrical neck portion, about 20 nm long and 20\u201325 nm in diameter. The neck portion was surrounded by a kind of cytoplasmic dense material, about 10 nm thick, reminiscent of a \u201ccollar.\u201d Thus, these pits are referred to as \u201ccollared pits.\u201d Similar kinds of pits were observed, although very rarely, in wild\u2010type flies at 19 and 30\u00b0C and in <jats:italic>shi<\/jats:italic> flies at 19\u00b0C. In addition, various degrees of vesicle depletion, and an increase in membranous structures (infoldings and cisternalike or tubulelike structures) often accompanied pit formation. The possibility that these pits are the result of a blocked step in the endocytotic process, which in turn causes vesicle depletion as exocytosis proceeds, is discussed.<\/jats:p>","DOI":"10.1002\/neu.480140305","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T02:51:02Z","timestamp":1104547862000},"page":"207-225","source":"Crossref","is-referenced-by-count":253,"title":["Possible temperature\u2010dependent blockage of synaptic vesicle recycling induced by a single gene mutation in <i>drosophila<\/i>"],"prefix":"10.1002","volume":"14","author":[{"given":"Toshio","family":"Kosaka","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuo","family":"Ikeda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,11]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1007\/BF01248567","article-title":"Coated vesicles in the pericardial cells of the aphid (Myzus persicae, Sulz)","volume":"59","author":"Bowers B.","year":"1964","journal-title":"Protoplasma"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1980.60.2.396"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-8166(72)80029-6"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.78.3.685"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00267238"},{"key":"e_1_2_1_7_1","first-page":"728","article-title":"Three temperature\u2010sensitive loci causing paralysis in Drosophila melanogaster","volume":"14","author":"Hall L.","year":"1972","journal-title":"Can. 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