{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T02:37:54Z","timestamp":1780454274624,"version":"3.54.1"},"reference-count":45,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2004,1,29]],"date-time":"2004-01-29T00:00:00Z","timestamp":1075334400000},"content-version":"vor","delay-in-days":4198,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Yeast"],"published-print":{"date-parts":[[1992,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We describe the isolation of mutants of the yeast <jats:italic>Pichia pastoris<\/jats:italic> that are deficient in peroxisome assembly (<jats:italic>pas<\/jats:italic>). These mutants of <jats:italic>P. pastoris<\/jats:italic> can be identified solely by their inability to grow on methanol and oleic acid, the utilization of which requires peroxisomal enzymes, and are defined by the absence of normal peroxisomes as judged by electron microscopy and biochemical fractionation experiments. These mutants are the result of genetic defects at single loci and represent at least eight different complementation groups. The isolation of <jats:italic>pas<\/jats:italic> mutants of <jats:italic>P. pastoris<\/jats:italic> by a simple screen for mutants unable to use methanol and oleic acid represents a significantly more efficient method for identification of <jats:italic>pas<\/jats:italic> mutants than is possible in other organisms. To exploit this advantage fully we also developed new reagents for the genetic and molecular manipulation of <jats:italic>P. pastoris<\/jats:italic>. These include a set of auxotropic strains with an essentialiy wild type genetic background, plasmids that act as <jats:italic>Escherichia coli\u2013P. pastoris<\/jats:italic> shuttle vectors, and genomic DNA libraries for isolation of <jats:italic>P. pastoris<\/jats:italic> genes by functional complementation of mutants or by nucleic acid hybridization. The availability of numerous <jats:italic>pas<\/jats:italic> mutants and the reagents necessary for their molecular analysis should lead to the isolation and characterization of genes involved in peroxisome assembly.<\/jats:p>","DOI":"10.1002\/yea.320080805","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T23:39:51Z","timestamp":1117323591000},"page":"613-628","source":"Crossref","is-referenced-by-count":179,"title":["Development of the yeast <i>Pichia pastoris<\/i> as a model organism for a genetic and molecular analysis of peroxisome assembly"],"prefix":"10.1002","volume":"8","author":[{"given":"Stephen J.","family":"Gould","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dannel","family":"McCollum","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allan P.","family":"Spong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John A.","family":"Heyman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suresh","family":"Subramani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,1,29]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","DOI":"10.1016\/S0021-9258(18)54483-5","article-title":"The carboxy\u2010terminal tripeptide Ala\u2010Lys\u2010Ile is essential for targeting Candida tropicalis trifunctional enzyme to yeast peroxisomes","volume":"266","author":"Aitchison J. 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