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We studied 71 punch biopsy skin samples of 35 patients with Creutzfeldt\u2013Jakob disease (CJD), including five assessed in vitam. The results confirmed the high value of skin prion RT\u2010QuIC for CJD diagnosis (89% sensitivity and 100% specificity) and support its use in clinical practice. Preliminary data based on a limited number of cases suggest that prion\u2010seeding activity in the skin varies according to the prion strain, being higher in sporadic CJD subtypes linked to the V2 strain (VV2 and MV2K) than in typical CJDMM1.<\/jats:p>","DOI":"10.1002\/acn3.51000","type":"journal-article","created":{"date-parts":[[2020,3,6]],"date-time":"2020-03-06T12:48:48Z","timestamp":1583498928000},"page":"559-564","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":68,"title":["Detection of prions in skin punch biopsies of Creutzfeldt\u2013Jakob disease patients"],"prefix":"10.1002","volume":"7","author":[{"given":"Angela","family":"Mammana","sequence":"first","affiliation":[{"name":"IRCCS Istituto delle Scienze Neurologiche di Bologna Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone","family":"Baiardi","sequence":"additional","affiliation":[{"name":"Department of Biomedical and 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