{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T18:23:00Z","timestamp":1759774980181,"version":"3.41.2"},"reference-count":31,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2020,9,30]],"date-time":"2020-09-30T00:00:00Z","timestamp":1601424000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/BTM\u2010MAT\/31210\/2017"],"award-info":[{"award-number":["PTDC\/BTM\u2010MAT\/31210\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010663","name":"H2020 European Research Council","doi-asserted-by":"publisher","award":["ERC\u20102014\u2010AdG\u2010669858"],"award-info":[{"award-number":["ERC\u20102014\u2010AdG\u2010669858"]}],"id":[{"id":"10.13039\/100010663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv. Biosys."],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A plethora of bioinspired cell\u2010laden hydrogels are being explored as building blocks that once assembled are able to create complex and highly hierarchical structures recapitulating the heterogeneity of living tissues. Yet, the resulting 3D bioengineered systems still present key limitations, mainly related with limited diffusion of essential molecules for cell survival, which dictates the failure of most strategies upon implantation. To maximize the hierarchical complexity of bioengineered systems, while simultaneously fully addressing the exchange efficiency of biomolecules, the high\u2010throughput fabrication of liquefied capsules is proposed using superhydrophobic\u2013superhydrophilic microarrays as platforms to produce the initial structures with high fidelity of geometry and size. The liquefied capsules are composed by i) a permselective multilayered membrane; ii) surface\u2010functionalized poly(\u03b5\u2010caprolactone) microparticles loaded into the liquefied core acting as cell adhesion sites; and iii) cells. It is demonstrated that besides the typical spherical liquefied capsules, it is also possible to obtain multi\u2010shaped blocks with high geometrical precision and efficiency. Importantly, the internal gelation approach used to produce such blocks does not jeopardize cell viability, evidencing the mild conditions of the proposed cell encapsulation technique. The proposed system is intended to be used as hybrid devices implantable using minimally invasive procedures for multiple tissue engineering applications.<\/jats:p>","DOI":"10.1002\/adbi.202000127","type":"journal-article","created":{"date-parts":[[2020,9,30]],"date-time":"2020-09-30T08:24:06Z","timestamp":1601454246000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Geometrically Controlled Liquefied Capsules for Modular Tissue Engineering Strategies"],"prefix":"10.1002","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5266-7657","authenticated-orcid":false,"given":"Sara","family":"Nadine","sequence":"first","affiliation":[{"name":"Department of Chemistry CICECO \u2013 Aveiro Institute of Materials University of Aveiro, Campus Universit\u00e1rio de Santiago  Aveiro 3810\u2010193 Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4976-7662","authenticated-orcid":false,"given":"S\u00f3nia G.","family":"Patr\u00edcio","sequence":"additional","affiliation":[{"name":"Department of Chemistry CICECO \u2013 Aveiro Institute of Materials University of Aveiro, Campus Universit\u00e1rio de Santiago  Aveiro 3810\u2010193 Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8178-6134","authenticated-orcid":false,"given":"Cristina C.","family":"Barrias","sequence":"additional","affiliation":[{"name":"i3S, Instituto De Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o Em Sa\u00fade Universidade Do Porto  Rua Alfredo Allen, 208 Porto 4200\u2010135 Portugal"},{"name":"INEB, Instituto De Engenharia Biom\u00e9dica Universidade Do Porto  Rua Alfredo Allen, 208 Porto 4200\u2010135 Portugal"},{"name":"ICBAS, Instituto De Ci\u00eancias Biom\u00e9dicas Abel Salazar Universidade Do Porto  Rua De Jorge Viterbo Ferreira, 228 Porto 4050\u2010313 Portugal"}]},{"given":"Insung S.","family":"Choi","sequence":"additional","affiliation":[{"name":"Center for Cell\u2010Encapsulation Research Department of Chemistry, KAIST  Daejeon 34141 South Korea"}]},{"given":"Michiya","family":"Matsusaki","sequence":"additional","affiliation":[{"name":"Division of Applied Chemistry Graduate School of Engineering Osaka University 2\u20101 Yamadaoka  Suita Osaka 565\u20100871 Japan"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1559-6295","authenticated-orcid":false,"given":"Clara R.","family":"Correia","sequence":"additional","affiliation":[{"name":"Department of Chemistry CICECO \u2013 Aveiro Institute of Materials University of Aveiro, Campus Universit\u00e1rio de Santiago  Aveiro 3810\u2010193 Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2342-3765","authenticated-orcid":false,"given":"Jo\u00e3o F.","family":"Mano","sequence":"additional","affiliation":[{"name":"Department of Chemistry CICECO \u2013 Aveiro Institute of Materials University of Aveiro, Campus Universit\u00e1rio de Santiago  Aveiro 3810\u2010193 Portugal"}]}],"member":"311","published-online":{"date-parts":[[2020,9,30]]},"reference":[{"key":"e_1_2_5_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2007.07.030"},{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201903975"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201701444"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt874"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201303233"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2018.07.015"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2016.12.020"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.105.001560"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201908061"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201570248"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.200801667"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm301833z"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtbio.2019.100009"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2010.0096"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep21883"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201600034"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5090\/ab3e16"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.2217\/nnm.14.175"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/admi.201400269"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2lc40921f"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2010.12.029"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.07.013"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201602350"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2011.12.034"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(02)00175-8"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr400531v"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2015.04.005"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2011.03.016"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2011.03.014"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/6737345"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201102545"}],"container-title":["Advanced Biosystems"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adbi.202000127","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/adbi.202000127","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adbi.202000127","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,3]],"date-time":"2023-09-03T10:40:37Z","timestamp":1693737637000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/adbi.202000127"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,30]]},"references-count":31,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2020,11]]}},"alternative-id":["10.1002\/adbi.202000127"],"URL":"https:\/\/doi.org\/10.1002\/adbi.202000127","archive":["Portico"],"relation":{},"ISSN":["2366-7478","2366-7478"],"issn-type":[{"type":"print","value":"2366-7478"},{"type":"electronic","value":"2366-7478"}],"subject":[],"published":{"date-parts":[[2020,9,30]]},"assertion":[{"value":"2020-05-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-30","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2000127"}}