{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,19]],"date-time":"2026-04-19T10:57:54Z","timestamp":1776596274202,"version":"3.51.2"},"reference-count":46,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,9,3]],"date-time":"2012-09-03T00:00:00Z","timestamp":1346630400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Adv Funct Materials"],"published-print":{"date-parts":[[2013,1,28]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The fabrication of hierarchical and bioactive self\u2010supporting membranes, which integrate physical and biomolecular elements, using a single\u2010step process that combines molecular self\u2010assembly with soft lithography is reported. A positively charged multidomain peptide (with or without the cell\u2010adhesive sequence arginine\u2010glycine\u2010aspartic acid\u2010serine (RGDS)) self\u2010assembles with hyaluronic acid (HA), an anionic biopolymer. Optimization of the assembling conditions enables the realization of membranes with well\u2010controlled and easily tunable features at multiple size scales including peptide sequence, building\u2010block co\u2010assembly, membrane thickness, bioactive epitope availability, and topographical pattern morphology. Membrane structure, morphology, and bioactivity are investigated according to temperature, assembly time, and variations in the experimental setup. Furthermore, to evaluate the physical and biomolecular signaling of the self\u2010assembled microfabricated membranes, rat mesenchymal stem cells are cultured on membranes exhibiting various densities of RGDS and different topographical patterns. Cell adhesion, spreading, and morphology are significantly affected by the surface topographical patterns and the different concentrations of RGDS. The versatility of the combined bottom\u2010up and top\u2010down fabrication processes described may permit the development of hierarchical macrostructures with precise biomolecular and physical properties and the opportunity to fine tune them with spatiotemporal control.<\/jats:p>","DOI":"10.1002\/adfm.201201065","type":"journal-article","created":{"date-parts":[[2012,9,3]],"date-time":"2012-09-03T04:45:37Z","timestamp":1346647537000},"page":"430-438","source":"Crossref","is-referenced-by-count":51,"title":["Co\u2010Assembled and Microfabricated Bioactive Membranes"],"prefix":"10.1002","volume":"23","author":[{"given":"Ana C.","family":"Mendes","sequence":"first","affiliation":[]},{"given":"Katherine H.","family":"Smith","sequence":"additional","affiliation":[]},{"given":"Esther","family":"Tejeda\u2010Montes","sequence":"additional","affiliation":[]},{"given":"Elisabeth","family":"Engel","sequence":"additional","affiliation":[]},{"given":"Rui L.","family":"Reis","sequence":"additional","affiliation":[]},{"given":"Helena S.","family":"Azevedo","sequence":"additional","affiliation":[]},{"given":"Alvaro","family":"Mata","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2012,9,3]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmat1339"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature08601"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchem.1123"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1154586"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1039\/b923903k"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.10.048"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature10513"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1039\/c1cs15064b"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.matsci.28.1.153"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmat2109"},{"key":"e_1_2_7_10_3","doi-asserted-by":"publisher","DOI":"10.1126\/science.276.5317.1401"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1039\/b819002j"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2009.05.023"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2011.09.005"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00383"},{"key":"e_1_2_7_14_3","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2006.01.044"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1359-0286(01)00005-5"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1020950022074"},{"key":"e_1_2_7_17_2","first-page":"389","volume":"2","author":"Mata A.","year":"2007","journal-title":"Int. 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