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Using mostly natural polymers and clean processes, it is possible to create macroporous monoliths with well\u2010defined porous networks, tuneable mechanical properties, and easy functionalization with a biomimetic ligand specific for Ab. Magnetic nanoparticles (MNPs) are embedded on the monolith network to confer a controlled magnetic response that facilitates and accelerates Ab recovery in the elution step. The hybrid monolithic systems prepared with agarose or chitosan\/poly(vinyl alcohol) (PVA) blends exhibit promising binding capacities of Abs directly from cell\u2010culture extracts (120 \u00b1 10 mg Ab g<jats:sup>\u22121<\/jats:sup> support) and controlled Ab magnetically\u2010assisted elution yielding 95 \u00b1 2% recovery. Moreover, a selective capture of mAbs directly from cell culture extracts is achieved yielding a final mAb preparation with 96% of purity.<\/jats:p>","DOI":"10.1002\/adfm.201400022","type":"journal-article","created":{"date-parts":[[2014,4,14]],"date-time":"2014-04-14T03:27:21Z","timestamp":1397446041000},"page":"4528-4541","source":"Crossref","is-referenced-by-count":22,"title":["Hybrid Monoliths for Magnetically\u2010Driven Protein Separations"],"prefix":"10.1002","volume":"24","author":[{"given":"Telma","family":"Barroso","sequence":"first","affiliation":[{"name":"REQUIMTE, Departamento de Qu\u00edmica, Faculdade de Ci\u00eancias e Tecnologia Universidade Nova de Lisboa  2829\u2013516 Caparica Portugal"}]},{"given":"Teresa","family":"Casimiro","sequence":"additional","affiliation":[{"name":"REQUIMTE, Departamento de Qu\u00edmica, Faculdade de Ci\u00eancias e Tecnologia Universidade Nova de Lisboa  2829\u2013516 Caparica Portugal"}]},{"given":"Ana M.","family":"Ferraria","sequence":"additional","affiliation":[{"name":"CQFM and IN, Instituto Superior T\u00e9cnico Universidade de Lisboa  1049\u2013001 Lisboa Portugal"}]},{"given":"F\u00e1bio","family":"Mattioli","sequence":"additional","affiliation":[{"name":"Center for Micro\u2010BioRobotics@SSSA Istituto Italiano di Tecnologia  Viale Rinaldo Piaggio 34 56025 Pontedera Italy"}]},{"given":"Ana","family":"Aguiar\u2010Ricardo","sequence":"additional","affiliation":[{"name":"REQUIMTE, Departamento de Qu\u00edmica, Faculdade de Ci\u00eancias e Tecnologia Universidade Nova de Lisboa  2829\u2013516 Caparica Portugal"}]},{"given":"Ana C. 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