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Nevertheless, the current vaccines are still lacking efficacy. Combination therapies, such as those integrating chemotherapy with immunotherapy, represent a powerful tool to potentially circumvent this drawback. Herein, injectable alginate cryogels loaded with granulocyte\u2010macrophage colony\u2010stimulating factor and cytosine\u2010phosphodiester\u2010guanine\u2010rich oligonucleotides, are combined with spermine\u2010modified acetalated dextran nanoparticles (Sp\u2010AcDEX NPs), loaded with p53 activator Nutlin\u20103a (Nut\u20103a) for combined chemoimmunotherapy. The Sp\u2010AcDEX NPs are successfully loaded into the alginate cryogels and released over time. Furthermore, the delivery of the NPs from the cryogel enhances their accumulation in tumor tissue following peritumoral injection. Nut\u20103a exerts toxicity towards the tumor cells and induces immunogenic cell death through the upregulation of surface calreticulin expression. Overall, this combination is a promising strategy to reduce cancer cell proliferation, induce immunogenic cell death, and accumulate drug payloads into the tumor. This approach may avoid cancer recurrence through the induction of in situ cancer vaccination mediated by antigens and danger signals released from the apoptotic cancer cells.<\/jats:p>","DOI":"10.1002\/adfm.201903686","type":"journal-article","created":{"date-parts":[[2019,6,27]],"date-time":"2019-06-27T05:31:26Z","timestamp":1561613486000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":62,"title":["Acetalated Dextran Nanoparticles Loaded into an Injectable Alginate Cryogel for Combined Chemotherapy and Cancer Vaccination"],"prefix":"10.1002","volume":"29","author":[{"given":"Tom\u00e1s","family":"Bauleth\u2010Ramos","sequence":"first","affiliation":[{"name":"Drug Research Program Division of Pharmaceutical Chemistry and Technology Faculty of Pharmacy University of Helsinki  FI\u201000014 Helsinki Finland"},{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade (I3S) University of Porto  Rua Alfredo Allen, 208 4200\u2010135 Porto Portugal"},{"name":"Instituto de Engenharia Biom\u00e9dica (INEB) University of Porto  Rua Alfredo Allen, 208 4200\u2010135 Porto Portugal"},{"name":"Instituto Ci\u00eancias Biom\u00e9dicas Abel Salazar (ICBAS) University of Porto  Rua Jorge Viterbo 228 4150\u2013180 Porto Portugal"},{"name":"John A. PaulsonSchool of Engineering and Applied Sciences Harvard University  Cambridge MA 02138 USA"}]},{"given":"Ting\u2010Yu","family":"Shih","sequence":"additional","affiliation":[{"name":"John A. PaulsonSchool of Engineering and Applied Sciences Harvard University  Cambridge MA 02138 USA"},{"name":"Wyss Institute for Biologically Inspired Engineering Harvard University  Cambridge MA 02138 USA"}]},{"given":"Mohammad\u2010Ali","family":"Shahbazi","sequence":"additional","affiliation":[{"name":"Drug Research Program Division of Pharmaceutical Chemistry and Technology Faculty of Pharmacy University of Helsinki  FI\u201000014 Helsinki Finland"},{"name":"Department of Pharmaceutical Nanotechnology School of Pharmacy Zanjan University of Medical Sciences  56184\u201045139 Zanjan Iran"}]},{"given":"Alexander J.","family":"Najibi","sequence":"additional","affiliation":[{"name":"John A. 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PaulsonSchool of Engineering and Applied Sciences Harvard University  Cambridge MA 02138 USA"},{"name":"Wyss Institute for Biologically Inspired Engineering Harvard University  Cambridge MA 02138 USA"}]},{"given":"Dongfei","family":"Liu","sequence":"additional","affiliation":[{"name":"Drug Research Program Division of Pharmaceutical Chemistry and Technology Faculty of Pharmacy University of Helsinki  FI\u201000014 Helsinki Finland"},{"name":"Helsinki Institute of Life Science (HiLIFE) University of Helsinki  FI\u201000014 Helsinki Finland"}]},{"given":"Pedro","family":"Granja","sequence":"additional","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade (I3S) University of Porto  Rua Alfredo Allen, 208 4200\u2010135 Porto Portugal"},{"name":"Instituto de Engenharia Biom\u00e9dica (INEB) University of Porto  Rua Alfredo Allen, 208 4200\u2010135 Porto Portugal"},{"name":"Instituto Ci\u00eancias Biom\u00e9dicas Abel Salazar (ICBAS) University of Porto  Rua Jorge Viterbo 228 4150\u2013180 Porto Portugal"}]},{"given":"H\u00e9lder A.","family":"Santos","sequence":"additional","affiliation":[{"name":"Drug Research Program Division of Pharmaceutical Chemistry and Technology Faculty of Pharmacy University of Helsinki  FI\u201000014 Helsinki Finland"},{"name":"Helsinki Institute of Life Science (HiLIFE) University of Helsinki  FI\u201000014 Helsinki Finland"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5763-7553","authenticated-orcid":false,"given":"Bruno","family":"Sarmento","sequence":"additional","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade (I3S) University of Porto  Rua Alfredo Allen, 208 4200\u2010135 Porto Portugal"},{"name":"Instituto de Engenharia Biom\u00e9dica (INEB) University of Porto  Rua Alfredo Allen, 208 4200\u2010135 Porto Portugal"},{"name":"CESPU Instituto de Investiga\u00e7\u00e3o e Forma\u00e7\u00e3o Avan\u00e7ada em Ci\u00eancias e Tecnologias da Sa\u00fade &amp; Instituto Universit\u00e1rio de Ci\u00eancias da Sa\u00fade  4585\u2010116 Gandra Portugal"}]},{"given":"David J.","family":"Mooney","sequence":"additional","affiliation":[{"name":"John A. 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