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Biomaterials that sequester endogenous GFs by affinity binding might circumvent such limitations and thus are being increasingly investigated. Here, molecularly imprinted nanoparticles (MINPs) are proposed as specific abiotic ligands for GFs. As a proof of concept, a conformational epitope of transforming growth factor\u2010\u03b23 (TGF\u2010\u03b23) is designed and surface imprinted onto polyacrylamide\u2010based nanoparticles by inverse microemulsion polymerization. It is found that, depending on the polymerization mixture composition, MINPs can recognize and preferentially bind TGF\u2010\u03b23, either in noncompetitive assays or from a complex human fluid (platelet lysate). Substrates functionalized with MINPs are then used for 2D culture of adipose\u2010derived stem cells. Remarkably, gene and protein expression profiles show a marked upregulation of SOX\u20109, suggesting activation of TGF\u2010\u03b23 signaling pathways without requiring supplementation with exogenous GF. Likewise, culturing these cells in pellets incorporating MINPs previously incubated with platelet lysate results in higher collagen II\u2010rich matrix deposition, compared to pellets incorporating non\u2010imprinted nanoparticles. In summary, results suggest MINPs can be used as cost\u2010effective, stable, and scalable alternative abiotic GF ligands to guide cell fate in TERM applications.<\/jats:p>","DOI":"10.1002\/adfm.202003934","type":"journal-article","created":{"date-parts":[[2020,10,20]],"date-time":"2020-10-20T11:15:05Z","timestamp":1603192505000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Epitope\u2010Imprinted Nanoparticles as Transforming Growth Factor\u2010\u03b23 Sequestering Ligands to Modulate Stem Cell Fate"],"prefix":"10.1002","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2764-4319","authenticated-orcid":false,"given":"Sim\u00e3o P. 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4805\u2010017 Portugal"},{"name":"ICVS\/3B's\u2013PT Government Associate Laboratory  Braga\/Guimar\u00e3es Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1035-2520","authenticated-orcid":false,"given":"Dominika","family":"Berdecka","sequence":"additional","affiliation":[{"name":"3B's Research Group I3Bs\u2014Research Institute on Biomaterials, Biodegradables and Biomimetics University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine AvePark \u2010 Parque de Ci\u00eancia e Tecnologia Zona Industrial da Gandra  Barco Guimar\u00e3es 4805\u2010017 Portugal"},{"name":"ICVS\/3B's\u2013PT Government Associate Laboratory  Braga\/Guimar\u00e3es Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7633-8890","authenticated-orcid":false,"given":"Margarida S.","family":"Miranda","sequence":"additional","affiliation":[{"name":"3B's Research Group I3Bs\u2014Research Institute on Biomaterials, Biodegradables and Biomimetics University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine AvePark \u2010 Parque de Ci\u00eancia e Tecnologia Zona Industrial da Gandra  Barco Guimar\u00e3es 4805\u2010017 Portugal"},{"name":"ICVS\/3B's\u2013PT Government Associate Laboratory  Braga\/Guimar\u00e3es Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2036-6291","authenticated-orcid":false,"given":"Manuela E.","family":"Gomes","sequence":"additional","affiliation":[{"name":"3B's Research Group I3Bs\u2014Research Institute on Biomaterials, Biodegradables and Biomimetics University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine AvePark \u2010 Parque de Ci\u00eancia e Tecnologia Zona Industrial da Gandra  Barco Guimar\u00e3es 4805\u2010017 Portugal"},{"name":"ICVS\/3B's\u2013PT Government Associate Laboratory  Braga\/Guimar\u00e3es Portugal"}]},{"given":"Nicholas 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