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As a unique self\u2010maintained and biocompatible 3D scaffold, the recently reported self\u2010feeding hydrogels with enzyme\u2010empowered degradation capacity have shown high biological performance in vitro and in vivo. To take advantage of all features of both PL and self\u2010feeding hydrogels, here UV responsive laminaran\u2010methacrylate (LamMA) and PL\u2010methacrylate (PLMA) derivatives plus glucoamylase (GA), which significantly improve the overall features of a 3D system, is coupled. This self\u2010sustaining hybrid hydrogel emerges as a unique scaffold due to the sustained delivery of glucose produced via enzymatic degradation of laminaran while granting the release of growth factors through the presence of PL. This biomaterial is applied to fabricate high\u2010throughput freestanding microgels with controlled geometric shapes. Furthermore, this multicomponent hybrid hydrogel is successfully implemented as the first reported glucose supplier bioink to manufacture intricate and precisely defined cell\u2010laden structures using a support matrix. Finally, such hydrogels are utilized as a proof of concept to serve as 3D in vitro cancer models, with the aim of recapitulating the tumor microenvironment.<\/jats:p>","DOI":"10.1002\/adfm.202214372","type":"journal-article","created":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T00:08:45Z","timestamp":1693872525000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["A Self\u2010Sustaining Hydrogels with Autonomous Supply of Nutrients and Bioactive Domains for 3D Cell Culture"],"prefix":"10.1002","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4740-9538","authenticated-orcid":false,"given":"Mehrzad","family":"Zargarzadeh","sequence":"first","affiliation":[{"name":"Department of Chemistry CICECO \u2013 Aveiro Institute of Materials University of Aveiro  3810\u2010193 Aveiro Portugal"}]},{"given":"Maria C.","family":"Gomes","sequence":"additional","affiliation":[{"name":"Department of Chemistry CICECO \u2013 Aveiro Institute of Materials University of Aveiro  3810\u2010193 Aveiro Portugal"}]},{"given":"S\u00f3nia G.","family":"Patr\u00edcio","sequence":"additional","affiliation":[{"name":"Department of Chemistry CICECO \u2013 Aveiro Institute of Materials University of Aveiro  3810\u2010193 Aveiro Portugal"}]},{"given":"Catarina A.","family":"Cust\u00f3dio","sequence":"additional","affiliation":[{"name":"Department of Chemistry CICECO \u2013 Aveiro Institute of Materials University of Aveiro  3810\u2010193 Aveiro Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2342-3765","authenticated-orcid":false,"given":"Jo\u00e3o F.","family":"Mano","sequence":"additional","affiliation":[{"name":"Department of Chemistry CICECO \u2013 Aveiro Institute of Materials University of Aveiro  3810\u2010193 Aveiro Portugal"}]}],"member":"311","published-online":{"date-parts":[[2023,9,4]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-02917-6"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-27744-0"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.progpolymsci.2011.07.003"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2018.10.009"},{"key":"e_1_2_8_5_1","first-page":"1561","volume-title":"Protein\u2010Based Hydrogels. 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