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This biomimicry potential can be expanded by combining dECM with key components of native tissues, such as glycosaminoglycans (GAGs). Yet, this combination remains highly challenging, often requiring complex non\u2010natural chemical modifications of dECM biomaterials. Taking advantage of naturally occurring tyrosine moieties, herein a universally applicable methodology is proposed for generating dECM\u2010Hyaluronic acid (HA)\u2010tyramine hydrogels. Enriching dECM with HA, is particularly relevant for modeling tumor\u2010stroma interplay in vitro. By using visible light crosslinking, pancreatic tissue\u2010specific dECM\u2010HA\u2010Tyr tumor\u2010stroma compartmentalyzed platforms are rapidly fabricated on\u2010demand, in superhydrophobic surfaces, while avoiding complex dECM chemical modifications. The generated dECM\u2010HA\u2010Tyr spherically shaped models are able to mimic the desmoplastic stromal environment and are employed for dual screening of pancreatic anti\u2010stromal and anti\u2010tumoral therapeutics. The tumor\u2010stroma models display higher susceptibility to gemcitabine following a first treatment with anti\u2010stroma therapeutics. The developed photo\u2010compartmentalized models ultimately allow to decouple the tumor and stroma compartment, thus enabling the inclusion of other stromal\/cancer cells. Moreover, the possibility of combining dECM with virtually any class of tyramine\u2010functionalized macromolecules via on\u2010demand photocrosslinking as herein proposed, extends the use of such hybrid dECM hydrogels.<\/jats:p>","DOI":"10.1002\/adfm.202305473","type":"journal-article","created":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T01:15:15Z","timestamp":1698282915000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Photo\u2010Compartmentalized Decellularized Matrix\u2010Hyaluronan Hybrid Units for Pancreatic Tumor\u2010Stroma Modeling"],"prefix":"10.1002","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4963-6267","authenticated-orcid":false,"given":"Maria V.","family":"Monteiro","sequence":"first","affiliation":[{"name":"Department of Chemistry CICECO\u2013Aveiro Institute of Materials University of Aveiro Campus Universit\u00e1rio de Santiago  Aveiro 3810\u2010193 Portugal"}]},{"given":"Margarida","family":"Henriques\u2010Pereira","sequence":"additional","affiliation":[{"name":"Department of Chemistry CICECO\u2013Aveiro Institute of Materials University of Aveiro Campus Universit\u00e1rio de Santiago  Aveiro 3810\u2010193 Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7391-3124","authenticated-orcid":false,"given":"Bruno M.","family":"Neves","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences and Institute of Biomedicine\u2010iBiMED University of Aveiro  Aveiro 3810\u2010193 Portugal"}]},{"given":"Iola D.","family":"Duarte","sequence":"additional","affiliation":[{"name":"Department of Chemistry CICECO\u2013Aveiro Institute of Materials University of Aveiro Campus Universit\u00e1rio de Santiago  Aveiro 3810\u2010193 Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0372-2493","authenticated-orcid":false,"given":"V\u00edtor M.","family":"Gaspar","sequence":"additional","affiliation":[{"name":"Department of 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