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However, it is challenging to assess and control their internal environment post\u2010production without compromising their structural integrity. This study explores liquid capsules with shells created from gelatin modified with hydroxypyridinone groups and coordinated with iron ions to enable access to and control over their internal content. Using glycerol as a cryoprotectant prevents ice crystal formation in gelatin\u2010derived hydrogel pores during storage at \u221220\u00a0\u00b0C. The hygroscopic properties provided by glycerol effectively preserve the structural and self\u2010healing features of the shell over time, supporting large\u2010scale production of off\u2010the\u2010shelf containers. As a proof\u2010of\u2010concept, the ability to manipulate the internal content, and real\u2010time analysis of internal pH, oxygen, and protein levels is shown. The nature of these capsules allows them to closely emulate the elasticity and self\u2010healing of natural cell membranes, enabling in situ modulation of the internal content without compromising the capsule structural integrity. These findings support the development of universal incubator units for in vitro studies, advancing bioreactors, sensors, and frameworks crucial for bioengineering microtissues across diverse applications.<\/jats:p>","DOI":"10.1002\/adfm.202425715","type":"journal-article","created":{"date-parts":[[2025,1,30]],"date-time":"2025-01-30T02:01:58Z","timestamp":1738202518000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Pierceable, Storable, and Manipulable Liquid Capsules for Precise Monitoring and Efficient Cargo Transport in Biotechnological Advances"],"prefix":"10.1002","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9815-7484","authenticated-orcid":false,"given":"Ana Rita","family":"Pinho","sequence":"first","affiliation":[{"name":"CICECO Department of Chemistry University of Aveiro Campus Universit\u00e1rio de Santiago  Aveiro 3810\u2010193 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