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Size exclusion chromatography analysis of the methacrylated gellan gum (GG\u2010MA) powder revealed that molecular weight is lower as compared to the non\u2010modified material, i.e., low acyl gellan gum. The water uptake and swelling of iGG\u2010MA and phGG\u2010MA hydrogels were investigated in phosphate\u2010buffered saline solution (pH 7.4). The biocompatibility of the hydrogels was firstly evaluated by producing cell\u2010laden hydrogels. The in vitro cells encapsulation study showed that lung fibroblast cells (L929 cells) and human intervertebral disc (hIVD) cells are viable when cultured within both hydrogels, up to 21 days of culturing. The iGG\u2010MA and phGG\u2010MA hydrogels were also subcutaneously implanted in Lewis rats for 10 and 18 days. Tissue response to the hydrogels implantation was determined by histological analysis (haematoxylin\u2010eosin staining). A thin fibrous capsule was observed around the implanted hydrogels. No necrosis, calcification, and acute inflammatory reaction were observed. The results presented in this study demonstrate that iGG\u2010MA and phGG\u2010MA hydrogels are stable in vitro and in vivo, support L929 and hIVD cells\u2019 encapsulation and viability, and were found to be well\u2010tolerated and non\u2010toxic in vivo.<\/jats:p>","DOI":"10.1002\/adhm.201200256","type":"journal-article","created":{"date-parts":[[2012,11,2]],"date-time":"2012-11-02T05:35:53Z","timestamp":1351834553000},"page":"568-575","source":"Crossref","is-referenced-by-count":99,"title":["Biocompatibility Evaluation of Ionic\u2010 and Photo\u2010Crosslinked Methacrylated Gellan Gum Hydrogels: In Vitro and In Vivo Study"],"prefix":"10.1002","volume":"2","author":[{"given":"Joana","family":"Silva\u2010Correia","sequence":"first","affiliation":[]},{"given":"Barbara","family":"Zavan","sequence":"additional","affiliation":[]},{"given":"Vincenzo","family":"Vindigni","sequence":"additional","affiliation":[]},{"given":"Tiago H.","family":"Silva","sequence":"additional","affiliation":[]},{"given":"Joaquim M.","family":"Oliveira","sequence":"additional","affiliation":[]},{"given":"Giovanni","family":"Abatangelo","sequence":"additional","affiliation":[]},{"given":"Rui L.","family":"Reis","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2012,11,2]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.trap.2009.05.001"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aanat.2010.12.001"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2007.12.011"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007632-198802000-00008"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.spinee.2010.02.005"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00586-008-0746-2"},{"key":"e_1_2_7_7_2","author":"Pereira D. 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