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In this work, a method is developed for the preparation of nanostructured hollow multilayers tubes combining LbL and template leaching. The aim is to produce hollow tubes based on polyelectrolyte multilayer films with tuned physical\u2010chemical properties and study their effects on cell behavior. The final tubular structures are characterized by differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), microscopy, swelling, and mechanical tests, including dynamic mechanical analysis (DMA) in physiological simulated conditions. It is found that more robust films could be produced upon chemical cross\u2010linking with genipin. In particular, the mechanical properties confirms the viscoelastic properties and a storage and young modulus about two times higher. The water uptake decreases from about 390% to 110% after the cross\u2010linking. The biological performance is assessed in terms of cell adhesion, viability, and proliferation. The results obtained with the cross\u2010linked tubes demonstrate that these are more suitable structures for cell adhesion and spreading. The results suggest the potential of these structures to boost the development of innovative tubular structures for tissue engineering approaches.<\/jats:p>","DOI":"10.1002\/adhm.201300265","type":"journal-article","created":{"date-parts":[[2013,8,26]],"date-time":"2013-08-26T05:22:48Z","timestamp":1377494568000},"page":"433-440","source":"Crossref","is-referenced-by-count":52,"title":["Nanostructured Hollow Tubes Based on Chitosan and Alginate Multilayers"],"prefix":"10.1002","volume":"3","author":[{"given":"Joana M.","family":"Silva","sequence":"first","affiliation":[{"name":"3Bs Research Group\u2014Biomaterials Biodegradables, and Biomimetics  AvePark, Zona Industrial, da Gandra S. 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