{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T01:09:46Z","timestamp":1777424986409,"version":"3.51.4"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,8,4]],"date-time":"2014-08-04T00:00:00Z","timestamp":1407110400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:p>Stable liquid marbles (LM) are produced by coating liquid droplets with a hydrophobic powder. The used hydrophobic powder is produced by fluorosi\u00adlanization of diatomaceous earth, used before to produce superhydrophobic structures. Here, the use of LM is proposed for high\u2010throughput drug screening on anchorage\u2010dependent cells. To provide the required cell adhesion sites inside the liquid environment of LM, surface\u2010modified poly(<jats:sc>l<\/jats:sc>\u2010lactic acid) microparticles are used. A simple method that takes advantage from LM appealing features is presented, such as the ability to inject liquid on LM without disrupting (self\u2010healing ability), and to monitor color changes inside of LM. After promoting cell adhesion, a cytotoxic screening test is performed as a proof of concept. Fe<jats:sup>3+<\/jats:sup> is used as a model cytotoxic agent and is injected on LM. After incubation, AlamarBlue reagent is injected and used to assess the presence of viable cells, by monitoring color change from blue to red. Color intensity is measured by image processing and the analysis of pictures takes using an ordinary digital camera. The proposed method is fully validated in counterpoint to an MTS (3\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)\u20105\u2010(3\u2010carbo\u200bxymethoxyphenyl)\u20102\u2010(4\u2010sulfophenyl)\u20102H\u2010te\u200btrazolium) colorimetric assay, a well\u2010known method used for the cytotoxicity assessment.<\/jats:p>","DOI":"10.1002\/adhm.201400310","type":"journal-article","created":{"date-parts":[[2014,8,4]],"date-time":"2014-08-04T17:44:02Z","timestamp":1407174242000},"page":"264-270","source":"Crossref","is-referenced-by-count":41,"title":["Liquid Marbles for High\u2010Throughput Biological Screening of Anchorage\u2010Dependent Cells"],"prefix":"10.1002","volume":"4","author":[{"given":"Nuno M.","family":"Oliveira","sequence":"first","affiliation":[{"name":"3B's Research Group \u2013 Biomaterials, Biodegradables and Biomimetics University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine  AvePark, Zona Industrial da Granja, S. Cl\u00e1udio do Barco 4806\u2013909 Caldas das Taipas Portugal"},{"name":"ICVS\/3B's \u2013 PT Government Associate Laboratory  Braga\/Guimar\u00e3es Portugal"}]},{"given":"Clara R.","family":"Correia","sequence":"additional","affiliation":[{"name":"3B's Research Group \u2013 Biomaterials, Biodegradables and Biomimetics University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine  AvePark, Zona Industrial da Granja, S. Cl\u00e1udio do Barco 4806\u2013909 Caldas das Taipas Portugal"},{"name":"ICVS\/3B's \u2013 PT Government Associate Laboratory  Braga\/Guimar\u00e3es Portugal"}]},{"given":"Rui L.","family":"Reis","sequence":"additional","affiliation":[{"name":"3B's Research Group \u2013 Biomaterials, Biodegradables and Biomimetics University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine  AvePark, Zona Industrial da Granja, S. Cl\u00e1udio do Barco 4806\u2013909 Caldas das Taipas Portugal"},{"name":"ICVS\/3B's \u2013 PT Government Associate Laboratory  Braga\/Guimar\u00e3es Portugal"}]},{"given":"Jo\u00e3o F.","family":"Mano","sequence":"additional","affiliation":[{"name":"3B's Research Group \u2013 Biomaterials, Biodegradables and Biomimetics University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine  AvePark, Zona Industrial da Granja, S. 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