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This study reports on the development of a highly versatile and upscalable method based on additive manufacturing for the fabrication of arrays of scaffolds, which are enclosed into individualized perfusion chambers. Devices containing eight scaffolds and their corresponding bioreactor chambers are simultaneously fabricated utilizing a dual extrusion additive manufacturing system. To demonstrate the versatility of the concept, the scaffolds, while enclosed into the device, are subsequently surface\u2010coated with a biomimetic calcium phosphate layer by perfusion with simulated body fluid solution. 96 scaffolds are simultaneously seeded and cultured with human osteoblasts under highly controlled bidirectional perfusion dynamic conditions over 4 weeks. Both coated and noncoated resulting scaffolds show homogeneous cell distribution and high cell viability throughout the 4 weeks culture period and CaP\u2010coated scaffolds result in a significantly increased cell number. The methodology developed in this work exemplifies the applicability of additive manufacturing as a tool for further automation of studies in the field of tissue engineering and regenerative medicine.<\/jats:p>","DOI":"10.1002\/adhm.201400591","type":"journal-article","created":{"date-parts":[[2015,2,26]],"date-time":"2015-02-26T10:17:12Z","timestamp":1424945832000},"page":"864-873","source":"Crossref","is-referenced-by-count":20,"title":["Additively Manufactured Device for Dynamic Culture of Large Arrays of 3D Tissue Engineered Constructs"],"prefix":"10.1002","volume":"4","author":[{"given":"Pedro F.","family":"Costa","sequence":"first","affiliation":[{"name":"3B's Research Group\u2010Biomaterials, Biodegradables and Biomimetics University of Minho, Avepark\u2010Zona Industrial da Gandra S. Cl\u00e1udio do Barco  4806\u201009 Caldas das Taipas, Guimar\u00e3es Portugal"},{"name":"ICVS\/3B's\u2010PT Government Associate Laboratory  Braga\/Guimar\u00e3es Portugal"}]},{"given":"Dietmar W.","family":"Hutmacher","sequence":"additional","affiliation":[{"name":"Institute of Health and Biomedical Innovation Queensland University of Technology  60 Musk Avenue Kelvin Grove QLD 4059 Australia"}]},{"given":"Christina","family":"Theodoropoulos","sequence":"additional","affiliation":[{"name":"Institute of Health and Biomedical Innovation Queensland University of Technology  60 Musk Avenue Kelvin Grove QLD 4059 Australia"}]},{"given":"Manuela E.","family":"Gomes","sequence":"additional","affiliation":[{"name":"3B's Research Group\u2010Biomaterials, Biodegradables and Biomimetics University of Minho, Avepark\u2010Zona Industrial da Gandra S. 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