{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T05:50:05Z","timestamp":1771912205085,"version":"3.50.1"},"reference-count":55,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2016,3,18]],"date-time":"2016-03-18T00:00:00Z","timestamp":1458259200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Portuguese Foundation for Science and Technology","award":["SFRH\/BD\/69529\/2010"],"award-info":[{"award-number":["SFRH\/BD\/69529\/2010"]}]},{"name":"project SUPRARELAX","award":["PTDC\/FIS\/115048\/2009\/FCOMP-01-0124-FEDER\/015340"],"award-info":[{"award-number":["PTDC\/FIS\/115048\/2009\/FCOMP-01-0124-FEDER\/015340"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:p>TGF\u2010\u03b23 is enzymatically immobilized by transglutaminase\u20102 action to poly(<jats:sc>l<\/jats:sc>\u2010lactic acid) microparticles coated with collagen II. Microparticles are then encapsulated with stem cells inside liquified spherical compartments enfolded with a permselective shell through layer\u2010by\u2010layer adsorption. Magnetic nanoparticles are electrostatically bound to the multilayered shell, conferring magnetic\u2010response ability. The goal of this study is to engineer a closed environment inside which encapsulated stem cells would undergo a self\u2010regulated chondrogenesis. To test this hypothesis, capsules are cultured in chondrogenic differentiation medium without TGF\u2010\u03b23. Their biological outcome is compared with capsules encapsulating microparticles without TGF\u2010\u03b23 immobilization and cultured in normal chondrogenic differentiation medium containing soluble TGF\u2010\u03b23. Glycosaminoglycans quantification demosntrates that similar chondrogenesis levels are achieved. Moreover, collagen fibrils resembling the native extracellular matrix of cartilage can be observed. Importantly, the genetic evaluation of characteristic cartilage markers confirms the successful chondrogenesis, while hypertrophic markers are downregulated. In summary, the engineered capsules are able to provide a suitable and stable chondrogenesis environment for stem cells without the need of TGF\u2010\u03b23 supplementation. This kind of self\u2010regulated capsules with softness, robustness, and magnetic responsive characteristics is expected to provide injectability and in situ fixation, which is of great advantage for minimal invasive strategies to regenerate cartilage.<\/jats:p>","DOI":"10.1002\/adhm.201600034","type":"journal-article","created":{"date-parts":[[2016,3,19]],"date-time":"2016-03-19T03:22:04Z","timestamp":1458357724000},"page":"1346-1355","source":"Crossref","is-referenced-by-count":33,"title":["A Closed Chondromimetic Environment within Magnetic\u2010Responsive Liquified Capsules Encapsulating Stem Cells and Collagen II\/TGF\u2010\u03b23 Microparticles"],"prefix":"10.1002","volume":"5","author":[{"given":"Clara R.","family":"Correia","sequence":"first","affiliation":[{"name":"3B's Research Group \u2013 Biomaterials Biodegradables and Biomimetics University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine  AvePark 4805\u2010017 Barco Guimar\u00e3es Portugal"},{"name":"ICVS\/3B's\u2010PT Government Associated Laboratory  Braga\/Guimaraes Portugal"}]},{"given":"Sara","family":"Gil","sequence":"additional","affiliation":[{"name":"3B's Research Group \u2013 Biomaterials Biodegradables and Biomimetics University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine  AvePark 4805\u2010017 Barco Guimar\u00e3es Portugal"},{"name":"ICVS\/3B's\u2010PT Government Associated Laboratory  Braga\/Guimaraes Portugal"}]},{"given":"Rui L.","family":"Reis","sequence":"additional","affiliation":[{"name":"3B's Research Group \u2013 Biomaterials Biodegradables and Biomimetics University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine  AvePark 4805\u2010017 Barco Guimar\u00e3es Portugal"},{"name":"ICVS\/3B's\u2010PT Government Associated Laboratory  Braga\/Guimaraes Portugal"}]},{"given":"Jo\u00e3o F.","family":"Mano","sequence":"additional","affiliation":[{"name":"3B's Research Group \u2013 Biomaterials Biodegradables and Biomimetics University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine  AvePark 4805\u2010017 Barco Guimar\u00e3es Portugal"},{"name":"ICVS\/3B's\u2010PT Government Associated Laboratory  Braga\/Guimaraes Portugal"}]}],"member":"311","published-online":{"date-parts":[[2016,3,18]]},"reference":[{"key":"e_1_2_6_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0929-6646(09)60039-5"},{"key":"e_1_2_6_2_1","doi-asserted-by":"crossref","first-page":"2526","DOI":"10.1002\/jbm.a.34147","volume":"100","author":"Ge Z.","year":"2012","journal-title":"J. Biomed. Mater. Res., Part A"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2010.81"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/14653240310004539"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0070-2153(03)58005-X"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201300342"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2013.0681"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tec.2010.0467"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0055451"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2015.09.033"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-011-1019-7"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2013.09.028"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2013.03.013"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2009.07.022"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.05.033"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2015.02.079"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2013.09.086"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2015.02.033"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmat2999"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2011.0606"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2013.09.002"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2013.04.017"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2014.01.023"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.injury.2011.05.035"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdrc.20167"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.2001.5278"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1014"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2007.08.026"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1039\/C2SM26784E"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm301833z"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep21883"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201303844"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1039\/B600460C"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2013.01.084"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.5b00292"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201400611"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2005.04.017"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-008-0190-y"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.43.30707"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V98.5.1567"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.136.5.1151"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.4.1057"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00428a032"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.148.4.825"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10529-013-1301-8"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2005.05.098"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2009.0398"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.17.4.2336"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.2006.12.2853"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmbbm.2012.03.006"},{"key":"e_1_2_6_51_1","doi-asserted-by":"crossref","first-page":"159","DOI":"10.3233\/BME-2010-0627","volume":"20","author":"Merceron C.","year":"2010","journal-title":"Bio\u2010Med. Mater. Eng."},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2474-15-309"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.04.053"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matlet.2013.03.058"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e02-02-0105"}],"container-title":["Advanced Healthcare Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fadhm.201600034","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fadhm.201600034","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.201600034","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T09:43:09Z","timestamp":1759743789000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adhm.201600034"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,3,18]]},"references-count":55,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2016,6]]}},"alternative-id":["10.1002\/adhm.201600034"],"URL":"https:\/\/doi.org\/10.1002\/adhm.201600034","archive":["Portico"],"relation":{},"ISSN":["2192-2640","2192-2659"],"issn-type":[{"value":"2192-2640","type":"print"},{"value":"2192-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,3,18]]}}}