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Here, a new methodology to produce enzymatically cross\u2010linked silk fibroin TCs is described, which takes advantage of the tyrosine groups present in silk structure that are known to allow the formation of a covalently cross\u2010linked hydrogel. Three different processing methods are used as a final step to modulate the properties of the silk\u2010based TCs. This approach allows to virtually adjust any characteristic of the final TCs. The final microstructure ranges from a nonporous to a highly porous network, allowing the TCs to be selectively porous to 4 kDa molecules, but not to human skin fibroblasts. Mechanical properties are dependent both on the processing method and thickness of the TCs. Bioactivity is observed after 30 days of immersion in simulated body fluid only for the TCs submitted to a drying processing method (50 \u00b0C). The in vivo study performed in mice demonstrates the good biocompatibility of the TCs. The enzymatically cross\u2010linked silk fibroin TCs are versatile and have adjustable characteristics that can be exploited in a variety of biomedical applications, particularly in guidance of peripheral nerve regeneration.<\/jats:p>","DOI":"10.1002\/adhm.201800186","type":"journal-article","created":{"date-parts":[[2018,7,12]],"date-time":"2018-07-12T10:26:08Z","timestamp":1531391168000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Tunable Enzymatically Cross\u2010Linked Silk Fibroin Tubular Conduits for Guided Tissue Regeneration"],"prefix":"10.1002","volume":"7","author":[{"given":"Cristiana R.","family":"Carvalho","sequence":"first","affiliation":[{"name":"3B's Research Group I3Bs\u2014Research Institute on Biomaterials Biodegradables and Biomimetics University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine AvePark, Parque de Ci\u00eancia e Tecnologia Zona Industrial da Gandra  4805\u2010017 Barco Guimar\u00e3es Portugal"},{"name":"ICVS\/3B's\u2013PT Government Associate Laboratory  Braga Guimar\u00e3es Portugal"},{"name":"The Discoveries Centre for Regenerative and Precision Medicine Headquarters at University of Minho, Avepark  4805\u2010017 Barco Guimar\u00e3es Portugal"}]},{"given":"Jo\u00e3o B.","family":"Costa","sequence":"additional","affiliation":[{"name":"3B's Research Group I3Bs\u2014Research Institute on Biomaterials Biodegradables and Biomimetics University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine AvePark, Parque de Ci\u00eancia e Tecnologia Zona Industrial da Gandra  4805\u2010017 Barco Guimar\u00e3es Portugal"},{"name":"ICVS\/3B's\u2013PT Government Associate Laboratory  Braga Guimar\u00e3es Portugal"},{"name":"The Discoveries Centre for Regenerative and Precision Medicine Headquarters at University of Minho, Avepark  4805\u2010017 Barco Guimar\u00e3es Portugal"}]},{"given":"Alain","family":"da Silva Morais","sequence":"additional","affiliation":[{"name":"3B's Research Group I3Bs\u2014Research Institute on Biomaterials Biodegradables and Biomimetics University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine AvePark, Parque de Ci\u00eancia e Tecnologia Zona Industrial da Gandra  4805\u2010017 Barco Guimar\u00e3es Portugal"},{"name":"ICVS\/3B's\u2013PT Government Associate Laboratory  Braga Guimar\u00e3es Portugal"}]},{"given":"Rita","family":"L\u00f3pez\u2010Cebral","sequence":"additional","affiliation":[{"name":"3B's Research Group I3Bs\u2014Research Institute on Biomaterials Biodegradables and Biomimetics University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine AvePark, Parque de Ci\u00eancia e Tecnologia Zona Industrial da Gandra  4805\u2010017 Barco Guimar\u00e3es Portugal"},{"name":"ICVS\/3B's\u2013PT Government Associate Laboratory  Braga Guimar\u00e3es Portugal"},{"name":"The Discoveries Centre for Regenerative and Precision Medicine Headquarters at University of Minho, Avepark  4805\u2010017 Barco Guimar\u00e3es Portugal"}]},{"given":"Joana","family":"Silva\u2010Correia","sequence":"additional","affiliation":[{"name":"3B's Research Group I3Bs\u2014Research Institute on Biomaterials Biodegradables and Biomimetics University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine AvePark, Parque de Ci\u00eancia e Tecnologia Zona Industrial da Gandra  4805\u2010017 Barco Guimar\u00e3es Portugal"},{"name":"ICVS\/3B's\u2013PT Government Associate Laboratory  Braga Guimar\u00e3es Portugal"}]},{"given":"Rui L.","family":"Reis","sequence":"additional","affiliation":[{"name":"3B's Research Group I3Bs\u2014Research Institute on Biomaterials Biodegradables and Biomimetics University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine AvePark, Parque de Ci\u00eancia e Tecnologia Zona Industrial da Gandra  4805\u2010017 Barco Guimar\u00e3es Portugal"},{"name":"ICVS\/3B's\u2013PT Government Associate Laboratory  Braga Guimar\u00e3es Portugal"},{"name":"The Discoveries Centre for Regenerative and Precision Medicine Headquarters at University of Minho, Avepark  4805\u2010017 Barco Guimar\u00e3es Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7052-8837","authenticated-orcid":false,"given":"J. 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