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Indeed, the depletion of macrophages during bone repair often culminates in disease scenarios. Inspired by the native dynamics between immune and skeletal systems, this work proposes a straightforward in vitro method to bioengineer biomimetic bone niches using biological waste. For that, liquefied and semipermeable reservoirs generated by electrohydrodynamic atomization and layer\u2010by\u2010layer techniques are developed to coculture umbilical cord\u2010derived human cells, namely monocyte\u2010derived macrophages, mesenchymal\u2010derived stromal cells (MSCs), and human umbilical vein endothelial cells (HUVECs). Poly(\u03b5\u2010caprolactone) microparticles are also added to the liquefied core to act as cell carriers. The fabricated microcapsules grant the successful development of viable microtissues, ensuring the high diffusion of bioactive factors. Interestingly, macrophages within the bioengineered microcapsules increase the release of osteocalcin, osteoprotegerin, and vascular endothelial growth factor. The cytokines profile variation indicates macrophages' polarization into a prohealing phenotype. Altogether, the incorporation of macrophages within the fabricated microcapsules allows to recreate an appropriate bone microenvironment for developing new bone mineralized microtissues. The proposed bioencapsulation protocol is a powerful self\u2010regulated system, which might find great applicability in bone tissue engineering based on bottom\u2010up approaches or disease modeling.<\/jats:p>","DOI":"10.1002\/adhm.202200651","type":"journal-article","created":{"date-parts":[[2022,7,29]],"date-time":"2022-07-29T04:54:51Z","timestamp":1659070491000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Liquefied Microcapsules Compartmentalizing Macrophages and Umbilical Cord\u2010Derived Cells for Bone Tissue Engineering"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5266-7657","authenticated-orcid":false,"given":"Sara","family":"Nadine","sequence":"first","affiliation":[{"name":"CICECO \u2010 Aveiro Institute of Materials Department of Chemistry University of Aveiro Campus Universit\u00e1rio de Santiago  Aveiro 3810\u2010193 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