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Among these scaffolds, multichannel hydrogels, characterized by meticulously designed micrometer\u2010scale channels, stand out as instrumental tools for guiding axonal growth and facilitating cellular interactions. This study explores the innovative application of human amniotic membranes modified with methacryloyl domains (AMMA) in neural stem cell (NSC) culture. AMMA hydrogels, possessing a tailored softness resembling the physiological environment, are prepared in the format of multichannel scaffolds to simulate native\u2010like microarchitecture of nerve tracts. Preliminary experiments on AMMA hydrogel films showcase their potential for neural applications, demonstrating robust adhesion, proliferation, and differentiation of NSCs without the need for additional coatings. Transitioning into the 3D realm, the multichannel architecture fosters intricate neuronal networks guiding neurite extension longitudinally. Furthermore, the presence of synaptic vesicles within the cellular arrays suggests the establishment of functional synaptic connections, underscoring the physiological relevance of the developed neuronal networks. This work contributes to the ongoing efforts to find ethical, clinically translatable, and functionally relevant approaches for regenerative neuroscience.<\/jats:p>","DOI":"10.1002\/adhm.202400522","type":"journal-article","created":{"date-parts":[[2024,7,11]],"date-time":"2024-07-11T10:31:39Z","timestamp":1720693899000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Amniotic Membrane\u2010Derived Multichannel Hydrogels for Neural Tissue Repair"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4478-4532","authenticated-orcid":false,"given":"Joana P.M.","family":"Sousa","sequence":"first","affiliation":[{"name":"TEMA \u2013 Centre for Mechanical Technology and Automation Department of Mechanical Engineering University of Aveiro  Aveiro 3810\u2010193 Portugal"},{"name":"CICECO \u2013 Department of Chemistry University of Aveiro Campus Universitario de Santiago  Aveiro 3810\u2010193 Portugal"}]},{"given":"In\u00eas A.","family":"Deus","sequence":"additional","affiliation":[{"name":"CICECO \u2013 Department of Chemistry University of Aveiro Campus Universitario de Santiago  Aveiro 3810\u2010193 Portugal"}]},{"given":"C\u00e1tia F.","family":"Monteiro","sequence":"additional","affiliation":[{"name":"CICECO \u2013 Department of Chemistry University of Aveiro Campus Universitario de Santiago  Aveiro 3810\u2010193 Portugal"}]},{"given":"Catarina A.","family":"Cust\u00f3dio","sequence":"additional","affiliation":[{"name":"CICECO \u2013 Department of Chemistry University of Aveiro Campus Universitario de Santiago  Aveiro 3810\u2010193 Portugal"},{"name":"Metatissue PCI \u00b7 Creative Science Park Aveiro Region  Via do Conhecimento \u00cdlhavo 3830\u2010352 Portugal"}]},{"given":"Jo\u00e3o","family":"Gil","sequence":"additional","affiliation":[{"name":"TEMA \u2013 Centre for Mechanical Technology and Automation Department of Mechanical Engineering University of Aveiro  Aveiro 3810\u2010193 Portugal"},{"name":"CDRSP \u2013 Centre for Rapid and Sustainable Product Development Polytechnic of Leiria  Marinha Grande 2430\u2010028 Portugal"},{"name":"INESC\u2010MN \u2013 INESC Microsistemas e Nanotecnologia  Rua Alves Redol 9 Lisbon 1000\u2010029 Portugal"}]},{"given":"Lina","family":"Papadimitriou","sequence":"additional","affiliation":[{"name":"Institute of Electronic Structure and Laser Foundation for Research and Technology\u2010Hellas (FORTH\u2010IESL)  Heraklion Greece"}]},{"given":"Anthi","family":"Ranella","sequence":"additional","affiliation":[{"name":"Institute of Electronic Structure and Laser Foundation for Research and Technology\u2010Hellas (FORTH\u2010IESL)  Heraklion Greece"}]},{"given":"Emmanuel","family":"Stratakis","sequence":"additional","affiliation":[{"name":"Institute of Electronic Structure and Laser Foundation for Research and Technology\u2010Hellas (FORTH\u2010IESL)  Heraklion Greece"}]},{"given":"Jo\u00e3o F.","family":"Mano","sequence":"additional","affiliation":[{"name":"CDRSP \u2013 Centre for Rapid and Sustainable Product Development Polytechnic of Leiria  Marinha Grande 2430\u2010028 Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7498-452X","authenticated-orcid":false,"given":"Paula A.A.P.","family":"Marques","sequence":"additional","affiliation":[{"name":"TEMA \u2013 Centre for Mechanical Technology and Automation Department of Mechanical Engineering University of Aveiro  Aveiro 3810\u2010193 Portugal"},{"name":"LASI \u2013 Intelligent Systems Associate Laboratory  Portugal"}]}],"member":"311","published-online":{"date-parts":[[2024,7,11]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2018.04.009"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-49067-3"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a005108"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-023-01477-6"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-11707-7"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2022.114069"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2014.04.064"},{"key":"e_1_2_8_8_1","first-page":"1","volume":"2302498","author":"Ross B. 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