{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T22:52:58Z","timestamp":1776120778985,"version":"3.50.1"},"reference-count":53,"publisher":"Wiley","issue":"32","license":[{"start":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T00:00:00Z","timestamp":1754870400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Cardiovascular mortality remains a major health challenge. Cardiomyocyte (CM)\u2010based tissue engineering (TE) offers promising alternatives for developing therapies via in vitro models. However, the immature phenotype of CM in engineered tissues hampers progress. Recent studies introduce conductive materials like graphene to enhance CM maturation, but conventional graphene synthesis suffers from complexity, toxicity, and low yield. Laser\u2010induced graphene (LIG) provides a sustainable, cost\u2010effective, eco\u2010friendly solution with efficient conductivity and biocompatibility. A LIG\u2010based substrate is bioengineered in this study, hypothesizing that its conductive, anisotropic properties promote CM maturation and mimic the native cardiac niche. LIG is fabricated using a CO\n                    <jats:sub>2<\/jats:sub>\n                    laser with Parylene\u2010C as a precursor. Stem cells (SCs) and SC\u2010derived embryoid bodies (EBs) are cultured on LIG substrates, and their viability, metabolic activity, morphology, and protein expression are evaluated through immunofluorescence and electron microscopy. Both SCs and EBs maintain viability and activity throughout the culture. Moreover, EB\u2010derived CM exhibit spontaneous contraction and express cardiac\u2010specific proteins, confirming functional differentiation on LIG matrices. This first report demonstrates that LIG substrates support SC culture and differentiation, highlighting their potential in developing refined in vitro cardiac models and advancing regenerative therapeutic strategies. The findings support LIG as a transformative advancement in TE.\n                  <\/jats:p>","DOI":"10.1002\/adhm.202502255","type":"journal-article","created":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T10:31:21Z","timestamp":1754908281000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Laser\u2010Induced Graphene: A Promising Conductive Platform for Cell Culture"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9123-4475","authenticated-orcid":false,"given":"Henrique","family":"Vaz\u00e3o de Almeida","sequence":"first","affiliation":[{"name":"i3N|CENIMAT Department of Materials Science NOVA School of Science and Technology and CEMOP\/UNINOVA  Campus de Caparica Caparica 2829\u2010516 Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9790-8335","authenticated-orcid":false,"given":"Jos\u00e9 Manuel","family":"In\u00e1cio","sequence":"additional","affiliation":[{"name":"Stem Cells and Development Laboratory iNOVA4Health NOVA Medical School|Faculty of Medical Sciences Universidade NOVA de Lisboa  Lisbon 1169\u2010056 Portugal"}]},{"given":"Cl\u00e1udia","family":"Pereira","sequence":"additional","affiliation":[{"name":"i3N|CENIMAT Department of Materials Science NOVA School of Science and Technology and CEMOP\/UNINOVA  Campus de Caparica Caparica 2829\u2010516 Portugal"},{"name":"The Danish National Research Foundation and Villum Foundation's Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics Department of Health Technology Technical University of Denmark  Building 345CDK\u20102800 Kgs. Lyngby Denmark"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8346-4198","authenticated-orcid":false,"given":"Tom\u00e1s","family":"Pinheiro","sequence":"additional","affiliation":[{"name":"i3N|CENIMAT Department of Materials Science NOVA School of Science and Technology and CEMOP\/UNINOVA  Campus de Caparica Caparica 2829\u2010516 Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6798-111X","authenticated-orcid":false,"given":"Tom\u00e1s","family":"Calmeiro","sequence":"additional","affiliation":[{"name":"i3N|CENIMAT Department of Materials Science NOVA School of Science and Technology and CEMOP\/UNINOVA  Campus de Caparica Caparica 2829\u2010516 Portugal"}]},{"given":"Ricardo","family":"Correia","sequence":"additional","affiliation":[{"name":"i3N|CENIMAT Department of Materials Science NOVA School of Science and Technology and CEMOP\/UNINOVA  Campus de Caparica Caparica 2829\u2010516 Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4217-3842","authenticated-orcid":false,"given":"Jo\u00e3o","family":"Coelho","sequence":"additional","affiliation":[{"name":"i3N|CENIMAT Department of Materials Science NOVA School of Science and Technology and CEMOP\/UNINOVA  Campus de Caparica Caparica 2829\u2010516 Portugal"},{"name":"Dpto. F\u00edsica de la Materia Condensada and Instituto de Ciencia de Materiales de Sevilla Universidad de Sevilla\u2010CSIC  Avenida Reina Mercedes s\/n Sevilla 41012 Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0847-7711","authenticated-orcid":false,"given":"Joana Vaz","family":"Pinto","sequence":"additional","affiliation":[{"name":"i3N|CENIMAT Department of Materials Science NOVA School of Science and Technology and CEMOP\/UNINOVA  Campus de Caparica Caparica 2829\u2010516 Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7384-0949","authenticated-orcid":false,"given":"Jos\u00e9 Ant\u00f3nio","family":"Belo","sequence":"additional","affiliation":[{"name":"Stem Cells and Development Laboratory iNOVA4Health NOVA Medical School|Faculty of Medical Sciences Universidade NOVA de Lisboa  Lisbon 1169\u2010056 Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1997-7669","authenticated-orcid":false,"given":"Rodrigo","family":"Martins","sequence":"additional","affiliation":[{"name":"i3N|CENIMAT Department of Materials Science NOVA School of Science and Technology and CEMOP\/UNINOVA  Campus de Caparica Caparica 2829\u2010516 Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4202-7047","authenticated-orcid":false,"given":"Elvira","family":"Fortunato","sequence":"additional","affiliation":[{"name":"i3N|CENIMAT Department of Materials Science NOVA School of Science and Technology and CEMOP\/UNINOVA  Campus de Caparica Caparica 2829\u2010516 Portugal"}]}],"member":"311","published-online":{"date-parts":[[2025,8,11]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2020.580744"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a014076"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13036-019-0185-0"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.111.251843"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a014027"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.117.305365"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41569-019-0331-x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2524"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2017.05.048"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2016.0027"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsabm.0c01490"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201909880"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.201901229"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-08-102906-0.00003-9"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-323-85671-3.00012-9"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1039\/D3TB00654A"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.7b08777"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202212190"},{"key":"e_1_2_9_19_1","volume-title":"Graphene Bioelectronics","author":"Tiwari A.","year":"2018"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00710-023-00816-3"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202205292"},{"key":"e_1_2_9_22_1","first-page":"51","volume":"259","author":"Devasena T.","year":"2021","journal-title":"Rev. Environ. Contam. Toxicol."},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1088\/2515-7639\/ab9522"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/nano7120414"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2018.08.058"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2013.01.001"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.5b04751"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12989-016-0120-1"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2014.03.021"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2022.105388"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xpro.2023.102327"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40820-020-00496-0"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201803621"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202402014"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms6714"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201805271"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.0c20270"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/admi.202101502"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/admt.202000630"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.2c09667"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.6b00654"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.7b00019"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0081156"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2016.0055"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1080\/09205063.2021.1958465"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s42823-022-00365-3"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00604-022-05610-0"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.629430"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-00226-5"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-87550-y"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/adsr.202400052"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1021\/ar300009f"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0205108"}],"container-title":["Advanced Healthcare Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202502255","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T14:16:33Z","timestamp":1766153793000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adhm.202502255"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,11]]},"references-count":53,"journal-issue":{"issue":"32","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1002\/adhm.202502255"],"URL":"https:\/\/doi.org\/10.1002\/adhm.202502255","archive":["Portico"],"relation":{},"ISSN":["2192-2640","2192-2659"],"issn-type":[{"value":"2192-2640","type":"print"},{"value":"2192-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,11]]},"assertion":[{"value":"2025-05-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02255"}}