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Although bio\u2010fabrication techniques provide diverse strategies to create these channels, many require sophisticated equipment and time\u2010consuming protocols. Herein, collagenase, a degrading agent for methacrylated gelatin hydrogels, and magnetic nanoparticles (MNPs) are combined and processed into enzymatically active spherical structures using a straightforward oil bath emulsion methodology. The generated microgels are then used to microfabricate channels within biomimetic hydrogels via a novel sculpturing approach that relied on the precise coupling of protein\u2010enzyme pairs (for controlled local degradation) and magnetic actuation (for directional control). Results show that the sculpting velocity can be tailored by adjusting the magnetic field intensity or concentration of MNPs within the microgels. Additionally, varying the magnetic field position or microgel size generated diverse trajectories and channels of different widths. This innovative technology improves the viability of encapsulated cells through enhanced medium transport, outperforming non\u2010sculpted hydrogels and offering new perspectives for hydrogel vascularization and drug\/biomolecule administration. Ultimately, this novel concept can help design fully controlled channels in hydrogels or soft materials, even those with complex tortuosity, in a single wireless top\u2010down biocompatible step.<\/jats:p>","DOI":"10.1002\/adma.202402988","type":"journal-article","created":{"date-parts":[[2024,8,14]],"date-time":"2024-08-14T02:55:56Z","timestamp":1723604156000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Magneto\u2010Enzymatic Microgels for Precise Hydrogel Sculpturing"],"prefix":"10.1002","volume":"36","author":[{"given":"Maria C.","family":"Mendes","sequence":"first","affiliation":[{"name":"Department of Chemistry CICECO \u2013 Aveiro Institute of Materials University of Aveiro Campus Universit\u00e1rio de Santiago  Aveiro 3810\u2010193 Portugal"}]},{"given":"Jo\u00e3o A.","family":"Pereira","sequence":"additional","affiliation":[{"name":"Department of Chemistry CICECO \u2013 Aveiro Institute of Materials University of Aveiro Campus Universit\u00e1rio de Santiago  Aveiro 3810\u2010193 Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5388-1732","authenticated-orcid":false,"given":"Ana S.","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Chemistry CICECO \u2013 Aveiro Institute of Materials University of Aveiro Campus Universit\u00e1rio de Santiago  Aveiro 3810\u2010193 Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2342-3765","authenticated-orcid":false,"given":"Jo\u00e3o F.","family":"Mano","sequence":"additional","affiliation":[{"name":"Department of Chemistry CICECO \u2013 Aveiro Institute of Materials University of Aveiro Campus Universit\u00e1rio de Santiago  Aveiro 3810\u2010193 Portugal"}]}],"member":"311","published-online":{"date-parts":[[2024,8,13]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-021-00533-1"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.6b00269"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom11060863"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202306765"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201302042"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.200802106"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.18063\/IJB.2017.01.008"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-420145-3.00009-2"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2014.04.091"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.04.001"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2014.00052"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2018.06.038"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.7b18535"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2015.02.024"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201400611"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2019.119664"},{"key":"e_1_2_8_17_1","first-page":"8","volume":"11","author":"Santos L. 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