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However, existing callus organoids generally lack a functional vascular network and an appropriate mechanical microenvironment, and their production limits scalability and uniformity. Here, we established a droplet\u2010based microfluidic platform for the high\u2010throughput generation of morphology\u2010dependent magnesium silicate\u2010engineered composite hydrogel microspheres, enabling the uniform construction of vascularized callus organoids. Importantly, morphology\u2010dependent magnesium silicate could regulate both the bulk mechanical performance and internal stress distribution of the composite hydrogels. In vitro, callus organoids modulated by magnesium silicate nanotubes significantly promoted chondrogenic and osteogenic differentiation, developed a stable endothelial network and mineralized structure. Furthermore, magnesium silicate nanotube\u2010engineered composite hydrogel (2NT\u2010GM) offered a favorable mechanical microenvironment for callus organoids, thereby activating the mechanotransduction\u2010related pathway for promoting the development of organoids. In a rat femoral condyle critical\u2010sized defect model, the vascularized callus organoids regulated by 2NT\u2010GM were further found to significantly promote vascular network formation, and facilitate bone regeneration at the bone defect center within 6 weeks of implantation. Together, this study suggests a promising strategy for bone tissue regeneration by developing vascularized callus organoids with the stimulation of inorganic silicate biomaterials.<\/jats:p>","DOI":"10.1002\/adma.75339","type":"journal-article","created":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T07:34:24Z","timestamp":1791617664000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Silicate Biomaterials\u2010Stimulated Vascularized Callus Organoids for Bone Tissue Regeneration"],"prefix":"10.1002","author":[{"given":"Shijie","family":"Cao","sequence":"first","affiliation":[{"name":"State Key Laboratory of High Performance Ceramics Shanghai Institute of Ceramics Chinese Academy of Sciences  Shanghai People's Republic of China"},{"name":"Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of Sciences  Beijing People's Republic of 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