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Antimicrobial coatings based on antimicrobial peptides (AMPs) are described as promising strategies to fight biomaterial\u2010associated infections. However, their efficiency in the presence of plasma and their effect on platelets adhesion\/activation, essential for blood contact applications, is not known. In this work, the AMP cecropin\u2013melittin (CM) is covalently immobilized onto polyurethane (PU) films envisaging a coating for intravascular catheters. Immobilization is done by dip\u2010coating of a layer of gold nanoparticles (Au NPs) functionalized with \uf8ffNH<jats:sub>2<\/jats:sub> and \uf8ffCOOH terminated polyethylene glycol (PEG). Surfaces characterized using scanning electron microscopy (SEM), X\u2010ray photoelectron spectroscopy (XPS), quartz crystal microbalance with dissipation (QCM\u2010D), and colorimetric assays reveal a stable and homogeneous coating distribution. CM coating significantly reduces <jats:italic>Staphylococcus epidermidis<\/jats:italic> adhesion to PU films (\u224880% in PBS). Its bactericidal activity is not affected in the presence of 1% human plasma (hPlasma) proteins with 65% reduction on viable bacteria comparing to PU. Moreover, CM coating is able to prevent platelet adhesion\/activation to PU films (\u224895% in PBS). This effect is also observed when surfaces are precoated with hPlasma. Overall, the developed antimicrobial coating demonstrates great potential to prevent bacterial infections on PU devices without instigating platelet adhesion\/activation.<\/jats:p>","DOI":"10.1002\/admi.201801390","type":"journal-article","created":{"date-parts":[[2018,10,29]],"date-time":"2018-10-29T04:25:30Z","timestamp":1540787130000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Cecropin\u2013Melittin Functionalized Polyurethane Surfaces Prevent <i>Staphylococcus epidermidis<\/i> Adhesion without Inducing Platelet Adhesion and Activation"],"prefix":"10.1002","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0285-6972","authenticated-orcid":false,"given":"Micaela M.","family":"Querido","sequence":"first","affiliation":[{"name":"i3S\u2010Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade Universidade do Porto  Rua Alfredo Allen 208 4200\u2010135 Porto Portugal"},{"name":"INEB\u2010Instituto de Engenharia Biom\u00e9dica Universidade do Porto  Rua Alfredo Allen 208 4200\u2010135 Porto Portugal"},{"name":"FEUP\u2010Faculdade de Engenharia da Universidade do Porto  Rua Dr. Roberto Frias 4200\u2010465 Porto Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4354-0256","authenticated-orcid":false,"given":"Helena P.","family":"Felgueiras","sequence":"additional","affiliation":[{"name":"i3S\u2010Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade Universidade do Porto  Rua Alfredo Allen 208 4200\u2010135 Porto Portugal"},{"name":"INEB\u2010Instituto de Engenharia Biom\u00e9dica Universidade do Porto  Rua Alfredo Allen 208 4200\u2010135 Porto Portugal"}]},{"given":"Akhilesh","family":"Rai","sequence":"additional","affiliation":[{"name":"Faculty of Medicine University of Coimbra  R. Larga 3004\u2010504 Coimbra Portugal"},{"name":"CNC\u2010Center for Neurosciences and Cell Biology University of Coimbra  R. Larga 3004\u2010504 Coimbra Portugal"}]},{"given":"Fab\u00edola","family":"Costa","sequence":"additional","affiliation":[{"name":"i3S\u2010Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade Universidade do Porto  Rua Alfredo Allen 208 4200\u2010135 Porto Portugal"},{"name":"INEB\u2010Instituto de Engenharia Biom\u00e9dica Universidade do Porto  Rua Alfredo Allen 208 4200\u2010135 Porto Portugal"}]},{"given":"Claudia","family":"Monteiro","sequence":"additional","affiliation":[{"name":"i3S\u2010Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade Universidade do Porto  Rua Alfredo Allen 208 4200\u2010135 Porto Portugal"},{"name":"INEB\u2010Instituto de Engenharia Biom\u00e9dica Universidade do Porto  Rua Alfredo Allen 208 4200\u2010135 Porto Portugal"}]},{"given":"In\u00eas","family":"Borges","sequence":"additional","affiliation":[{"name":"i3S\u2010Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade Universidade do Porto  Rua Alfredo Allen 208 4200\u2010135 Porto Portugal"},{"name":"INEB\u2010Instituto de Engenharia Biom\u00e9dica Universidade do Porto  Rua Alfredo Allen 208 4200\u2010135 Porto Portugal"}]},{"given":"Diana","family":"Oliveira","sequence":"additional","affiliation":[{"name":"i3S\u2010Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade Universidade do Porto  Rua Alfredo Allen 208 4200\u2010135 Porto Portugal"},{"name":"INEB\u2010Instituto de Engenharia Biom\u00e9dica Universidade do Porto  Rua Alfredo Allen 208 4200\u2010135 Porto Portugal"},{"name":"FEUP\u2010Faculdade de Engenharia da Universidade do Porto  Rua Dr. Roberto Frias 4200\u2010465 Porto Portugal"}]},{"given":"Lino","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Faculty of Medicine University of Coimbra  R. 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